Invitrogen

Caveolin 3 Recombinant Rabbit Monoclonal Antibody (23GB3040)

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他10个Caveolin 3抗体

抗体检测数据 (3)
高级验证 (1)

Caveolin 3 Antibody in Immunocytochemistry (ICC/IF)

Caveolin 3 Antibody in Immunocytochemistry (ICC/IF)

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Caveolin 3 Antibody (MA5-53330) in ICC/IF

Immunocytochemistry analysis of Caveolin 3 in H9c2 cells. Nuclei were stained blue with DAPI; Caveolin 3 recombinant monoclonal antibody (Product # MA5-53330) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Caveolin 3 Antibody in Immunocytochemistry (ICC/IF)

Caveolin 3 Antibody in Western Blot (WB)

Caveolin 3 Antibody in Flow Cytometry (Flow)

Caveolin 3 Antibody

Product Image Gallery

Caveolin 3 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Caveolin 3 in H9c2 cells. Nuclei were stained blue with DAPI; Caveolin 3 recombinant monoclonal antibody (Product # MA5-53330) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Caveolin 3 Antibody in Western Blot (WB) — Western Blot analysis of Caveolin 3. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Caveolin 3 recombinant monoclonal antibody (Product # MA5-53330) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.

Caveolin 3 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of Caveolin 3 in H9c2 cells using Caveolin 3 recombinant monoclonal antibody (Product # MA5-53330) at a dilution of 1:2,000. Green, isotype control; red, Caveolin 3.

Caveolin 3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Caveolin 3 shRNA and decrease in signal intensity was observed in Western Blotting using Anti-Caveolin 3 Recombinant Rabbit Monoclonal Antibody (23GB3040) (Product # MA5-53330) at a dilution of 1:5,000. Secondary antibody used was a goat anti-rabbit (HRP) at a dilution of 1:20,000. {KD}

产品信息

MA5-53330

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

-

免疫细胞化学 (ICC/IF)

1:1,000

-

流式细胞分析 (Flow)

1:2,000

-

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293F

分类

Recombinant Monoclonal

类型

Antibody

克隆号

23GB3040

抗原

A synthesized peptide derived from Caveolin-3 (1-60AA).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

靶标信息

Caveolin-3 (CAV3) acts as a scaffolding protein within the caveolar membrane. It interacts directly with G-protein alpha subunits and can functionally regulate their activity. Caveolin-3 may also regulate voltage-gated potassium channels. It also plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress. Caveolin-3 also mediates the recruitment of CAVIN2 and CAVIN3 proteins to the caveolae. Mutations in the gene can result in HyperCKmia, rippling muscle disease 2, cardiomyopathy, long QT syndrome 9, sudden infant death syndrome, and myopathy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CAV; Caveolin-3; M-caveolin; MGC126100; MGC126101; MGC126129

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基因别名: CAV3; LGMD1C; LQT9; VIP-21; VIP21

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UniProt ID: (Human) P56539, (Rat) P51638

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Entrez Gene ID: (Human) 859, (Rat) 29161

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calcium channel regulator activity protein binding protein C-terminus binding sodium channel regulator activity potassium channel inhibitor activity protein complex binding protein complex scaffold alpha-tubulin binding ion channel binding nitric-oxide synthase binding connexin binding enzyme binding scaffold/adaptor protein

参与通路

plasma membrane repair regulation of heart rate negative regulation of protein kinase activity triglyceride metabolic process endocytosis muscle contraction plasma membrane organization actin filament organization positive regulation of cytosolic calcium ion concentration muscle organ development myoblast fusion regulation of heart contraction protein localization positive regulation of cell proliferation negative regulation of cardiac muscle hypertrophy positive regulation of myotube differentiation regulation of skeletal muscle contraction cell growth regulation of transforming growth factor beta receptor signaling pathway cell differentiation positive regulation of microtubule polymerization cytoplasmic microtubule organization membrane raft organization T-tubule organization detection of muscle stretch regulation of signal transduction by receptor internalization regulation of membrane potential glucose homeostasis cholesterol homeostasis negative regulation of MAP kinase activity negative regulation of MAPK cascade negative regulation of cell size muscle cell cellular homeostasis negative regulation of nitric-oxide synthase activity regulation of nerve growth factor receptor activity nucleus localization regulation of protein kinase B signaling negative regulation of calcium ion transport cardiac muscle cell development regulation of cardiac muscle contraction negative regulation of sarcomere organization regulation of ventricular cardiac muscle cell membrane repolarization heart trabecula formation regulation of ventricular cardiac muscle cell membrane depolarization regulation of branching involved in mammary gland duct morphogenesis negative regulation of cell growth involved in cardiac muscle cell development caveola assembly protein localization to plasma membrane ventricular cardiac muscle cell action potential establishment of protein localization to plasma membrane regulation of calcium ion import regulation of p38MAPK cascade regulation of membrane depolarization during cardiac muscle cell action potential negative regulation of membrane depolarization during cardiac muscle cell action potential negative regulation of potassium ion transmembrane transporter activity regulation of calcium ion transmembrane transporter activity negative regulation of potassium ion transmembrane transport negative regulation of protein localization to cell surface positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process regulation of sodium ion transmembrane transporter activity positive regulation of caveolin-mediated endocytosis myotube differentiation regulation of calcium ion transport