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Application
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Cdc5L Antibody (702831) in ICC/IF
For immunofluorescence analysis,HeLa cells were fixed and permeabilized for detection of endogenous CDC5L using Anti-CDC5L Recombinant Rabbit Monoclonal Antibody (Product # 702831, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000).Panel a) shows representative cells that were stained for detection and localization of CDC5L protein (green), panel b is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). P... View More
产品信息
702831
产品规格
种属反应
Human,
Mouse
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
22H12L1
抗原
Protein corresponding to human CDC5L [aa1-aa200]
偶联物
Unconjugated
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2734799
产品详细信息
This antibody is predicted to react with Monkey, Rabbit, Cat, Horse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
The protein encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CDC5 cell division cycle 5-like; Cdc5-like protein; Cdc5-related protein; Cell division cycle 5-like protein; cell division cycle 5-related protein; dJ319D22.1 (CDC5-like protein); hCDC5; Pombe cdc5-related protein
基因别名: 1200002I02Rik; AA408004; CDC5; CDC5-LIKE; CDC5L; CEF1; dJ319D22.1; KIAA0432; PCDC5RP
UniProt ID: (Human) Q99459, (Mouse) Q6A068
Entrez Gene ID: (Human) 988, (Mouse) 71702
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II transcription factor recruiting
transcription corepressor binding
DNA binding
protein binding
protein phosphatase 1 binding
protein kinase binding
leucine zipper domain binding
sequence-specific DNA binding
transcription regulatory region DNA binding
poly(A) RNA binding
WD40-repeat domain binding
chromatin binding
RNA binding
protein domain specific binding
mitotic cell cycle
mRNA splicing, via spliceosome
DNA repair
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cell differentiation
cellular response to fibroblast growth factor stimulus
cellular response to interleukin-2
signal transduction involved in DNA damage checkpoint
cellular response to wortmannin
cellular response to nerve growth factor stimulus
cellular response to prolactin
regulation of transcription, DNA-templated
mRNA processing
cellular response to DNA damage stimulus
cell cycle
RNA splicing
