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Claudin 18 Antibody (38-8100) in IHC (P)
Immunohistochemistry analysis of Claudin-18 (Mid) showing staining in the membrane and cytoplasm of paraffin-embedded human stomach tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 mi... View More
产品信息
38-8100
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide derived from an internal region of the human Claudin-18 protein, which differs from mouse by one conservative amino acid replacement.
偶联物
形式
Liquid
浓度
0.25 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2533383
靶标信息
Claudin-18 is a tight junction protein expressed as lung-and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene. The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor. A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human. Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein. Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells. These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and stomach. Claudin-18 expression has also been reported in the inner ear.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Claudin-18; surfactant associated 5; surfactant associated protein J; surfactant, pulmonary associated protein J
基因别名: CLDN18; SFTA5; SFTPJ; UNQ778/PRO1572
UniProt ID: (Human) P56856, (Mouse) P56857
Entrez Gene ID: (Human) 51208, (Mouse) 56492
