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Application
FIGURE: 1 / 2
Claudin 6 Antibody (PA5-120728) in WB
Western blot analysis of Claudin 6 in extracts of OVCAR3 cells. Samples were incubated with Claudin 6 Polyclonal antibody (Product # PA5-120728) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-120728
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 121-220 of human Claudin 6 (NP_067018.2).
Conjugate
Unconjugated
Form
Liquid
Concentration
1.564 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914300
Product Specific Information
Positive test controls include: OVCAR3, Rat brain.
Immunogen sequence: SGIVFVISGV LTLIPVCWTA HAIIRDFYNP LVAEAQKREL GASLYLGWAA SGLLLLGGGL LCCTCPSGGS QGPSHYMARY STSAPAISRG PSEYPTKNYV
Target Information
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. This gene encodes a component of tight junction strands, which is a member of the claudin family. The protein is an integral membrane protein and is one of the entry cofactors for hepatitis C virus. The gene methylation may be involved in esophageal tumorigenesis. This gene is adjacent to another family member CLDN9 on chromosome 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Claudin-6; Claudin6; CLD6; Skullin; tight junction molecule claudin 6
Gene Aliases: AL024037; CLDN6; UNQ757/PRO1488
UniProt ID: (Human) P56747, (Mouse) Q9Z262
Entrez Gene ID: (Human) 9074, (Mouse) 54419, (Rat) 287098
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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