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Connexin 43 Antibody (PA5-32778) in IHC (P)
Immunohistochemistry analysis of Connexin 43 in human testis, seminiferous tubules and Leydig cells. Samples were incubated with Connexin 43 polyclonal antibody (Product # PA5-32778). Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
产品信息
PA5-32778
产品规格
种属反应
Bovine,
Dog,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic 16 amino acid peptide from C-terminus of human GJA1 / Connexin 43.
偶联物
Unconjugated
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
0.1% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2550240
产品详细信息
Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Hamster, Elephant, Panda, Rabbit, Pig, Opossum, Guinea pig (100%); Bat, Horse, Chicken, Lizard, Xenopus (94%); Trout, Salmon (88%); Zebrafish, Sea anemone, Nematode (81%).
For IHC(P), use heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
靶标信息
Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: alpha 1 connexin; connexin 43; Connexin-43; Cx-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein; gap junction membrane channel protein alpha 1; gap junction protein; gap junction protein connexin 43; gap junction protein, alpha 1, 43 kD (connexin 43); gap junction protein, alpha 1, 43kDa; gap junction protein, alpha 1, 43kDa (connexin 43); ODD; ODOD; SDTY3; Syndactyly type III; Vascular smooth muscle connexin-43; vascular smooth muscle connexin43
基因别名: AU042049; AVSD3; AW546267; CMDR; Cnx43; connexin43; CX43; Cx43alpha1; Cxn-43; EKVP; Gja-1; GJA1; GJAL; HLHS1; HSS; Npm1; ODDD; PPKCA
UniProt ID: (Rabbit) Q6TYA7, (Human) P17302, (Bovine) P18246, (Mouse) P23242, (Rat) P08050
signal transducer activity
receptor binding
gap junction channel activity
protein binding
ion transmembrane transporter activity
SH3 domain binding
PDZ domain binding
beta-tubulin binding
connexin binding
gap junction channel activity involved in cardiac conduction electrical coupling
scaffold protein binding
gap junction channel activity involved in cell communication by electrical coupling
tubulin binding
gap junction hemi-channel activity
beta-catenin binding
protein domain specific binding
transmembrane transporter activity
gap junction
osteoblast differentiation
in utero embryonic development
neuron migration
negative regulation of endothelial cell proliferation
heart looping
regulation of heart rate
epithelial cell maturation
lens development in camera-type eye
chronic inflammatory response
positive regulation of glomerular filtration
endothelium development
atrial ventricular junction remodeling
transport
apoptotic process
muscle contraction
signal transduction
positive regulation of cytosolic calcium ion concentration
cell-cell signaling
heart development
adult heart development
response to pH
response to glucose
vascular transport
positive regulation of gene expression
negative regulation of gene expression
cell communication by chemical coupling
cell communication by electrical coupling
positive regulation of cell communication by chemical coupling
ATP transport
gap junction assembly
negative regulation of cell growth
regulation of bone mineralization
positive regulation of insulin secretion
ion transmembrane transport
response to fluid shear stress
embryonic digit morphogenesis
positive regulation of I-kappaB kinase/NF-kappaB signaling
skeletal muscle tissue regeneration
response to peptide hormone
positive regulation of osteoblast differentiation
positive regulation of protein catabolic process
positive regulation of striated muscle tissue development
positive regulation of vasoconstriction
positive regulation of vasodilation
regulation of bone remodeling
blood vessel morphogenesis
neuron projection morphogenesis
protein oligomerization
regulation of calcium ion transport
negative regulation of cardiac muscle cell proliferation
milk ejection
regulation of ventricular cardiac muscle cell membrane repolarization
regulation of atrial cardiac muscle cell membrane depolarization
regulation of ventricular cardiac muscle cell membrane depolarization
negative regulation of wound healing
cellular response to mechanical stimulus
atrial cardiac muscle cell action potential
cell communication by electrical coupling involved in cardiac conduction
negative regulation of DNA biosynthetic process
regulation of bicellular tight junction assembly
positive regulation of behavioral fear response
response to ischemia
spermatogenesis
response to wounding
cellular response to platelet-derived growth factor stimulus
regulation of apoptotic process
bone remodeling
transmembrane transport
bone development
positive regulation of smooth muscle cell chemotaxis
microtubule-based transport
establishment of mitotic spindle orientation
cell communication
regulation of heart contraction
negative regulation of cell proliferation
negative regulation of gonadotropin secretion
cellular protein localization
embryonic heart tube development
negative regulation of trophoblast cell migration
positive regulation of morphogenesis of an epithelium
positive regulation of mesodermal cell differentiation
positive regulation of stem cell proliferation
epicardial cell to mesenchymal cell transition
cell-cell junction organization
regulation of blood vessel remodeling
cardiac conduction
establishment of epithelial cell polarity
