Invitrogen

Cullin 3 Polyclonal Antibody

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Cullin 3 Antibody (PA5-95767) in WB

Western blot analysis of Cullin 3 in extracts of various cell lines. Samples were incubated with Cullin 3 Polyclonal antibody (Product # PA5-95767) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

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产品信息

PA5-95767

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫细胞化学 (ICC/IF)

1:50-1:200

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 569-768 of human Cullin 3 (NP_0035811)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.28 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2807569

产品详细信息

Positive Samples: HeLa, U-251MG, Jurkat, PC-12, Mouse brain, Mouse spleen, Rat brain; Cellular Location: Golgi apparatus, Nucleus

Immunogen sequence: KEDGSEVGVG GAQVTGSNTR KHILQVSTFQ MTILMLFNNR EKYTFEEIQQ ETDIPERELV RALQSLACGK PTQRVLTKEP KSKEIENGHI FTVNDQFTSK LHRVKIQTVA AKQGESDPER KETRQKVDDD RKHEIEAAIV RIMKSRKKMQ HNVLVAEVTQ QLKARFLPSP VVIKKRIEGL IEREYLARTP EDRKVYTYVA

靶标信息

Cullin 3 is a member of the family of human cullin genes (CUL 1, 2, 3, 4a, 4b, and 5) homologous to the S. cerevisiae cdc 53 gene. CUL 1 forms a complex with human p19 skp1 and F box protein p45 skp2. Together with cdc 53 (Known as Cul A), as three subunits, they form a ubiquitin ligase-controlling proteolysis of G1 cell cycle regulatory proteins. In contrast to CUL 1, CUL 2 does not associate with p19 skp1 and p45 skp2. Instead, CUL 2 forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongain C, Elongin B and VHL. The function of this association remains unknown.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。