Invitrogen

Cullin 3 Recombinant Rabbit Monoclonal Antibody (7B6)

Cullin 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Cullin 3 Antibody (MA5-49887) in IHC (P)

Immunohistochemistry analysis with a Cullin 3 monoclonal antibody (Product # MA5-49887) using a dilution of 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was ... View More

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产品信息

MA5-49887

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

7B6

抗原

A synthesized peptide derived from human Cullin 3

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.35 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C or -80°C if preferred

运输条件

Wet ice

RRID

AB_3092805

靶标信息

Cullin 3 is a member of the family of human cullin genes (CUL 1, 2, 3, 4a, 4b, and 5) homologous to the S. cerevisiae cdc 53 gene. CUL 1 forms a complex with human p19 skp1 and F box protein p45 skp2. Together with cdc 53 (Known as Cul A), as three subunits, they form a ubiquitin ligase-controlling proteolysis of G1 cell cycle regulatory proteins. In contrast to CUL 1, CUL 2 does not associate with p19 skp1 and p45 skp2. Instead, CUL 2 forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongain C, Elongin B and VHL. The function of this association remains unknown.

仅用于科研。不用于诊断过程。未经明确授权不得转售。