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Invitrogen
Cyclin B1 Monoclonal Antibody (V152)
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
图: 1 / 16
Cyclin B1 Antibody (MA1-155) in ICC/IF
Immunofluorescent analysis of Cyclin B1 (green) showing staining in the in the cytoplasm and nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cyclin B1 monoclonal antibody (Product # MA1-1... View More
产品信息
MA1-155
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse,
Non-human primate,
Rat,
Hamster,
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
V152
抗原
Hamster Cyclin B1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS with 30% glycerol, 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2536863
产品详细信息
MA1-155 detects Cyclin B1 in mammalian samples. MA1-155 has been successfully used in Immunohistochemistry (paraffin), Immunofluorescence, Immunoprecipitation, Flow Cytometry and Western Blot procedures. Immunoprecipitation and Western Blot analysis with MA1-155 show the accumulation of a prominent band at ~52 kDa in camptothecin and hydroxyurea treated cells. MA1-155 also detects additional unknown bands at ~35 and ~75 kDa. In Immunofluorescence applications, MA1-155 shows cyclin B1 co-localization with microtubules, whereas MA1-156 shows cyclin B2 staining consistent with the Golgi region.
MA1-155 specifically detects Cyclin B1 in in whole cell lysates, but will also cross-react with purified recombinant human Cyclin B2 in Western Blot applications.
靶标信息
Cyclins bind to and regulate the activity of the Cyclin dependent protein kinases (CDKs). Cyclin B1 or CCNB1 is a regulatory protein involved in mitosis. It is essential for the control of the cell cycle at the G2/M transition. Cyclin B1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. Cyclin B1 is not ubiquitinated during G2/M phase, resulting in its steady accumulation during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B1 is required for cell cycle progression. Cyclin B1 is overexpressed in various cancers, including breast, prostate, and non-small cell lung cancer.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: cyclin B1; cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; OTTHUMP00000221981; OTTHUMP00000223023
基因别名: Ccn-2; CCNB; CCNB1; Ccnb1-rs1; Ccnb1-rs13; Cycb; Cycb-4; Cycb-5; CycB1; Cycb1-rs1; I79_013589
UniProt ID: (Human) P14635, (Chinese hamster) Q08301, (Rat) P30277, (Mouse) P24860
Entrez Gene ID: (Human) 891, (Chinese hamster) 100770842, (Rat) 25203, (Mouse) 268697
G2/M transition of mitotic cell cycle
oocyte maturation
in utero embryonic development
negative regulation of protein phosphorylation
protein complex assembly
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
mitotic spindle organization
mitotic nuclear envelope disassembly
mitotic metaphase plate congression
spermatogenesis
response to mechanical stimulus
negative regulation of gene expression
anaphase-promoting complex-dependent catabolic process
positive regulation of mRNA 3'-end processing
positive regulation of histone phosphorylation
tissue regeneration
response to drug
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
positive regulation of mitotic cell cycle
response to DDT
positive regulation of fibroblast proliferation
digestive tract development
cell division
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
regulation of cell cycle
positive regulation of attachment of spindle microtubules to kinetochore
ventricular cardiac muscle cell development
positive regulation of cardiac muscle cell proliferation
regulation of chromosome condensation
cellular response to iron(III) ion
cellular response to fatty acid
cellular response to organic cyclic compound
cellular response to hypoxia
regulation of mitotic cell cycle spindle assembly checkpoint
histone H3-S10 phosphorylation involved in chromosome condensation
protein phosphorylation
cell cycle
mitotic nuclear division
positive regulation of cell cycle
