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Cyclophilin A Antibody (PA5-80920) in WB
Western Blot using Cyclophilin A Polyclonal Antibody (Product # PA5-80920) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: NIH-3T3 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 18 kDa. Observed band size: 15 kDa. (We are unsure of the identity of these extra bands).
产品信息
PA5-80920
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human Cyclophilin A protein (Met1-Glu165)
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2788184
产品详细信息
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human Cyclophilin A.
靶标信息
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cyclophilin; Cyclophilin A; Cyclosporin A-binding protein; CyP A; EC 5.2.1.8; epididymis secretory sperm binding protein Li 69p; HGNC:9253; MGC117158; MGC12404; MGC23397; Peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A (cyclophilin A); PPIase A; rotamase; Rotamase A; T cell cyclophilin
基因别名: CYPA; CYPH; HEL-S-69p; PPIA
UniProt ID: (Human) P62937
Entrez Gene ID: (Human) 5478
protein peptidyl-prolyl isomerization
RNA-dependent DNA biosynthetic process
protein folding
viral life cycle
uncoating of virus
fusion of virus membrane with host plasma membrane
virion assembly
viral release from host cell
entry into host cell
lipid particle organization
regulation of viral genome replication
positive regulation of viral genome replication
positive regulation of protein secretion
leukocyte migration
establishment of integrated proviral latency
