Invitrogen

Cytochrome C Monoclonal Antibody (6H2), eBioscience™

3个已发表图片

4 篇参考文献

查看其他47个Cytochrome C抗体

抗体检测数据 (1)
已发表图片 (3)

Cytochrome C Antibody in Flow Cytometry (Flow)

Cytochrome C Antibody in Flow Cytometry (Flow)

图: 1 / 4

Cytochrome C Antibody (14-6601-82) in Flow

Intracellular staining of HeLa cells with Anti-Cytochrome C FITC. Appropriate isotype controls were used (open histogram). Total cells were used for analysis.

Cytochrome C Antibody in Flow Cytometry (Flow)

Cytochrome C Antibody in Immunocytochemistry (ICC/IF)

Cytochrome C Antibody in Immunohistochemistry (IHC)

Cytochrome C Antibody in Flow Cytometry (Flow)

Product Image Gallery

Cytochrome C Antibody in Flow Cytometry (Flow) — Intracellular staining of HeLa cells with Anti-Cytochrome C FITC. Appropriate isotype controls were used (open histogram). Total cells were used for analysis.

Cytochrome C Antibody in Immunocytochemistry (ICC/IF) — 10.1371/journal.pntd.0001306.g001 Figure 1 Demonstration of characteristic membrane and cytoplasmic features of apoptosis in developing embryos of S.digitata . Developing embryos harvested from adult S.digitata worms were analyzed with a flow cytometer [BD FACS Calibur] using Dot plots and Histogram plots. [ A ] Dot plots for embryonic stages showing 3 distinct clusters of populations: R-1 representing microfilariae while R-2 and R-3 representing eggs-early and late embryonic stages respectively. Apoptosis was studied with different assays either by gating respective populations in the Dot plots - for 3 different populations of embryonic stages, individually or without gating - for the embryonic stages all together. [ B ] Overlaid histograms show phosphatidyl serine exposure on microfilariae and eggs by Annexin-V-PE staining after treatment with 10 uM [ Red ] or 50 uM [Green] of Plumbagin in comparison to untreated controls [Blue] [ C ] Dot Plots revealing depolarization of mitochondria in embryonic stages and its reversal by NAC are shown using Mitoscreen JC-1 staining. The percentage of events in the upper gate [R5] and lower gate [R6] represent population of embryonic stages having normal and depolarized mitochondria respectively. [ D ] Confocal microscopic images of untreated embryos showing punctate staining and Plumbagin treated embryos showing diffusely cytoplasmic staining for Cytochrome-c respectively. [ E ] Overlaid histogram shows increased cytosolic presence of Cy

Cytochrome C Antibody in Immunohistochemistry (IHC) — Figure 5 Demonstration of Cytochrome-c-CED-4 interaction in S.digitata and docking of Cytochrome-c and CED-4 of C.elegans. [ A ] Confocal images of untreated control [upper panel] or Plumbagin treated [lower panel] late embryonic stages demonstrating enhanced colocalization of Cytochrome-c and CED-4 after 24 hr Plumbagin treatment are shown. Regions of colocalization are highlighted as white patches using Image J software. Fluorescence intensity line profile/colocalization profile analysis for both the labeled proteins-Cytochrome-c and CED-4 in control as well as apoptotic embryos, revealing enhanced cololocalization of these proteins in the later are shown [ B ] Interaction of Cytochrome-c of C.elegans [ Magenta ] with alpha/beta [P-loop] ATP binding domain [ Blue ] of CED-4 is shown.

Cytochrome C Antibody in Flow Cytometry (Flow) — Figure 3 Immature thymocytes from ADA-deficient mice show increased expression of apoptotic markers. ( A ) Western blot analysis of protein lysates of thymi from P14 untreated mice (lane 1-6) and 3-month-PEG-ADA-treated mice (lane 7-8). As positive control for cleaved caspase-3 staining, unfractionated thymocytes were stimulated in vitro with the apoptosis-inducer etoposide (line 9). Anti-GAPDH stain was used as protein loading control. Full-length blots are presented in Supplementary Fig. 11 . ( B ) FACS analysis of MACS-enriched Lin neg thymocytes from untreated mice at P14. DN4 (Lin neg , CD25 - ) and DN2 + DN3 (Lin neg , CD25 + ) populations are gated. ( C ) Intracellular staining for cleaved caspase-3 of cell populations shown in (B). Unfractionated Lin neg thymocytes were stimulated in vitro with etoposide as apoptosis positive control. ( D ) Intracellular staining for cytochrome C of cell populations shown in (B). FACS plots in (C) and (D) are representative of two replicate experiments (n = 4, 2).

产品信息

14-6601-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

-

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

-

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

Assay-Dependent

查看2篇已发表文章 2篇已发表文章

免疫沉淀 (IP)

Assay-Dependent

-

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

6H2

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_468174

产品详细信息

Description: The 6H2 antibody reacts with the native form of mouse, human, and rat cytochrome c.

Applications Reported: The 6H2 antibody has been reported for use in intracellular flow cytometric analysis, and immunoprecipitation. (Fluorochrome conjugated 6H2 is recommended for use in intracellular flow cytometry.).

Applications Tested: The 6H2 antibody has been tested by intracellular flow cytometric analysis. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Inhibition of antiapoptotic BCL-2 proteins with ABT-263 induces fibroblast apoptosis, reversing persistent pulmonary fibrosis.

JCI insight

Cooley JC,Javkhlan N,Wilson JA,Foster DG,Edelman BL,Ortiz LA,Schwartz DA,Riches DW,Redente EF

Published figure using Cytochrome C monoclonal antibody (Product # 14-6601-82) in Flow Cytometry

Wed Feb 08 00:00:00 EST 2023

Metabolite and thymocyte development defects in ADA-SCID mice receiving enzyme replacement therapy.

Scientific reports

Moretti FA,Giardino G,Attenborough TCH,Gkazi AS,Margetts BK,la Marca G,Fairbanks L,Crompton T,Gaspar HB

Published figure using Cytochrome C monoclonal antibody (Product # 14-6601-82) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

Caspase dependent programmed cell death in developing embryos: a potential target for therapeutic intervention against pathogenic nematodes.

PLoS neglected tropical diseases

Mohapatra AD,Kumar S,Satapathy AK,Ravindran B

Published figure using Cytochrome C monoclonal antibody (Product # 14-6601-82) in Immunofluorescence

Thu Sep 01 00:00:00 EDT 2011

Caspase dependent programmed cell death in developing embryos: a potential target for therapeutic intervention against pathogenic nematodes.

PLoS neglected tropical diseases

Mohapatra AD,Kumar S,Satapathy AK,Ravindran B

Published figure using Cytochrome C monoclonal antibody (Product # 14-6601-82) in Immunofluorescence

Thu Sep 01 00:00:00 EDT 2011

生物信息学

蛋白别名: Cytochrome c; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; hc1

查看更多收起

基因别名: CYC; CYCS; CYCSA; HCS; THC4

查看更多收起

UniProt ID: (Human) P99999, (Mouse) P62897, (Rat) P62898

查看更多收起

Entrez Gene ID: (Human) 54205, (Mouse) 13063, (Rat) 25309

查看更多收起

protein serine/threonine phosphatase activity protein binding heme binding electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity metal ion binding electron carrier activity iron ion binding lipid binding

参与通路

response to reactive oxygen species mitochondrial electron transport, ubiquinol to cytochrome c mitochondrial electron transport, cytochrome c to oxygen protein dephosphorylation mitochondrion organization activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c cellular respiration intrinsic apoptotic signaling pathway apoptotic DNA fragmentation apoptotic process hydrogen peroxide metabolic process ATP synthesis coupled electron transport response to ischemia response to oxidative stress negative regulation of hydrogen peroxide biosynthetic process glial cell apoptotic process positive regulation of cellular respiration