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Invitrogen
Cytokeratin 20 Polyclonal Antibody
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Cytokeratin 20 Antibody (PA5-32464) in ICC/IF
Immunofluorescence analysis of Cytokeratin 20 was performed using HT-29 and SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 20 Polyclonal Antibody (Product # PA5-32464) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degr... View More
产品信息
PA5-32464
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to C-terminus of human keratin 20
偶联物
形式
Liquid
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.6, with 1% BSA
内含物
0.1% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2549933
产品详细信息
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is colon adenocarcinoma.
靶标信息
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CK-20; cytokeratin 20; Cytokeratin-20; K20; keratin 20, type I; Keratin, type I cytoskeletal 20; Keratin-20; MGC35423; Protein IT
基因别名: CD20; CK-20; CK20; K20; KRT20; KRT21
UniProt ID: (Human) P35900
Entrez Gene ID: (Human) 54474
