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Invitrogen
Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (SA35-03)
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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Cytokeratin 20 Antibody (MA5-31979) in ICC/IF
Immunofluorescence analysis of KRT20 was performed using HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (Product # MA5-31979) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and t... View More
产品信息
MA5-31979
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Rat
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SA35-03
抗原
Synthetic peptide within Human Cytokeratin 20 aa 375-424
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2809273
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CK-20; CK-21; cytokeratin 20; cytokeratin 21; Cytokeratin-20; Cytokeratin-21; K20; keratin 20, type I; Keratin, type I cytoskeletal 20; Keratin-20; MGC35423; Protein IT
基因别名: CD20; CK-20; CK20; K20; KRT20; KRT21
UniProt ID: (Human) P35900, (Rat) P25030
Entrez Gene ID: (Human) 54474, (Rat) 286912
