Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
DARPP-32 Polyclonal Antibody
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
图: 1 / 4
DARPP-32 Antibody (720203) in ICC/IF
Immunofluorescence analysis of DARPP-32 was performed using 70% confluent log phase SK-O-V3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with DARPP-32 Polyclonal Antibody (Product # 720203) at 1:250 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555 (Product # A32794)... View More
产品信息
720203
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Protein corresponding to Human PPP1R1B (aa 1-204)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 30% glycerol, 0.1% BSA
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2633201
产品详细信息
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Pig, Horse and Cat
靶标信息
DARPP-32 is a dopamine (DA) and AMP-regugated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regugation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: DARPP-32; dopamine and cAMP-regulated neuronal phosphoprotein 32; Dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32; FLJ20940; IPPD; neuronal phosphoprotein DARPP-32; OTTHUMP00000164275; OTTHUMP00000164276; PPR1B; protein pho; Protein phosphatase 1 regulatory subunit 1B
基因别名: AU040756; DARPP-32; DARPP32; PPP1R1B
UniProt ID: (Human) Q9UD71, (Rat) Q6J4I0, (Mouse) Q60829
Entrez Gene ID: (Human) 84152, (Rat) 360616, (Mouse) 19049
protein kinase inhibitor activity
cAMP-dependent protein kinase inhibitor activity
protein phosphatase inhibitor activity
protein serine/threonine phosphatase inhibitor activity
protein binding
protein phosphatase type 1 regulator activity
D1 dopamine receptor binding
D2 dopamine receptor binding
D3 dopamine receptor binding
D4 dopamine receptor binding
D5 dopamine receptor binding
phosphatase inhibitor
response to amphetamine
transcription, DNA-templated
signal transduction
negative regulation of female receptivity
visual learning
intracellular signal transduction
negative regulation of protein serine/threonine kinase activity
negative regulation of protein kinase activity
negative regulation of catalytic activity
