Invitrogen

DGAT2 Polyclonal Antibody

DGAT2 Antibody in Immunocytochemistry (ICC/IF)

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DGAT2 Antibody (PA5-103785) in ICC/IF

Immunofluorescent analysis of DGAT2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and DGAT2 polyclonal antibody (Product # PA5-103785) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

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产品信息

PA5-103785

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:3,000

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免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human DGAT2(Accession Q96PD7), corresponding to amino acid residues N155-R205.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2853118

产品详细信息

Antibody detects endogenous levels of total DGAT2.

靶标信息

Acyl-coenzyme A:diacylglycerol acyltransferase, also known as DGAT1 and ARGP1, is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT1 catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT1 plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism, such as intestinal fat absorption, lipoprotein assembly, adipose tissue form-ation and lactation. DGAT2, which has no homology to DGAT1, differs from DGAT1 in that its activity has been shown to be inhibited by MgCl in an in vitro assay. DGAT2 is expressed primarily in liver and white adipose tissue, which suggests that it plays an important role in mammalian triglyceride metabolism.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Accumulation of cholesterol, triglycerides and ceramides in hepatocellular carcinomas of diethylnitrosamine injected mice.

Lipids in health and disease

Haberl EM,Pohl R,Rein-Fischboeck L,Höring M,Krautbauer S,Liebisch G,Buechler C

PA5-103785 was used in Western Blot to show that ceramides mediate the anti-cancer effects of different chemotherapeutic drugs and restoration of ceramide levels is effective against hepatocellular carcinoma (HCC).

Sun Oct 10 00:00:00 UTC 2021

Ferroptotic cell death triggered by conjugated linolenic acids is mediated by ACSL1.

Nature communications

Beatty A,Singh T,Tyurina YY,Tyurin VA,Samovich S,Nicolas E,Maslar K,Zhou Y,Cai KQ,Tan Y,Doll S,Conrad M,Subramanian A,Bayır H,Kagan VE,Rennefahrt U,Peterson JR

PA5-103785 was used in Western Blot to identify conjugated linoleates including α-eleostearic acid (αESA) as ferroptosis inducers.

Wed Apr 14 00:00:00 UTC 2021

生物信息学

蛋白别名: Acyl-CoA retinol O-fatty-acyltransferase; ARAT; diacylglycerol acyltransferase 2; Diacylglycerol O-acyltransferase 2; diacylglycerol O-acyltransferase homolog 2; diacylglycerol O-acyltransferase-like protein 2; Diglyceride acyltransferase 2; DKFZp686A15125; retinol O-fatty-acyltransferase

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基因别名: 0610010B06Rik; ARAT; DGAT-2; DGAT2; GS1999FULL; HMFN1045; UNQ738/PRO1433

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UniProt ID: (Human) Q96PD7, (Rat) Q5FVP8, (Mouse) Q9DCV3

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Entrez Gene ID: (Human) 84649, (Rat) 252900, (Mouse) 67800

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功能

2-acylglycerol O-acyltransferase activity diacylglycerol O-acyltransferase activity protein homodimerization activity retinol O-fatty-acyltransferase activity transferase activity transferase activity, transferring acyl groups transferase activity, transferring acyl groups other than amino-acyl groups acyltransferase

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