Invitrogen

DHX9 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (10)
高级验证 (2)

DHX9 Antibody in Immunocytochemistry (ICC/IF)

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DHX9 Antibody (PA5-55754) in ICC/IF

Immunofluorescence analysis of DHX9 was performed using A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DHX9 Polyclonal Antibody (Product # PA5-55754) at 4 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:200... View More

DHX9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DHX9 Antibody in RNA Immunoprecipitation (RIP)

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DHX9 Antibody — Antibody specificity was demonstrated by siRNA mediated KD of target protein. A-431 cells were transfected with DHX9 siRNA and reduction of signal was observed in Western Blot using DHX9 Polyclonal Antibody (Product # PA5-55754). {KD}

产品信息

PA5-55754

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

免疫组化（石蜡） (IHC (P))

1:200-1:500

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

免疫沉淀 (IP)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human DHX9. Recombinant protein control fragment (Product #RP-94224).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.05 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2640597

产品详细信息

Immunogen sequence: PHLALKAENN SEVGASGYGV PGPTWDRGAN LKDYYSRKEE QEVQATLESE EVDLNAGLHG NWTLENAKAR LNQYFQKEKI QGEYKYTQVG PDHNRSF

Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 94%.

靶标信息

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Involvement of DHX9/YB-1 complex induced alternative splicing of Krüppel-like factor 5 mRNA in phenotypic transformation of vascular smooth muscle cells.

American journal of physiology. Cell physiology

Huan W,Zhang J,Li Y,Zhi K

PA5-55754 was used in Immunoprecipitation to understand the molecular mechanisms underlying phenotypic transformation of vascular smooth muscle cells in the development of aortic dissection disease.

Thu Aug 01 00:00:00 EDT 2019

生物信息学

蛋白别名: ATP-dependent RNA helicase A; D-E-A-D (aspartate-glutamate-alanine-aspartate) box polypeptide 9; DEAD (aspartate-glutamate-alanine-aspartate) box polypeptide 9; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9; DEAH (Asp-Glu-Ala-His) box helicase 9; DEAH (Asp-Glu-Ala-His) box polypeptide 9; DEAH box protein 9; DExH-box helicase 9; FLJ17406; Leukophysin; LKP; mHEL-5; NDH II; Nuclear DNA helicase II; OTTHUMP00000033287; RHA; RNA helicase; RNA helicase A

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基因别名: AI326842; DDX9; DHX9; HEL-5; LKP; mHEL-5; NDH2; NDHII; RHA

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UniProt ID: (Human) Q08211, (Mouse) O70133

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Entrez Gene ID: (Human) 1660, (Mouse) 13211, (Rat) 304859

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