Invitrogen

DOG-1 Recombinant Rabbit Monoclonal Antibody (SP31)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2个已发表图片

6 篇参考文献

查看其他34个DOG-1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

DOG-1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

DOG-1 Antibody (MA5-16358) in ICC/IF

Immunofluorescence analysis of DOG-1 was performed using 70% confluent log phase PC-3 and DU 145 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. PC-3 cells were labeled with DOG-1 Monoclonal Antibody (Product # MA5-16358) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More

DOG-1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

DOG-1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of DOG-1 was observed in PC-3, HT-29, HCT 116 and COLO 205 cells in comparison to DU 145, HEK-293 cells using Anti-DOG-1 Monoclonal Antibody (SP31) (Product # MA5-16358) in Western blot. {RE}

产品信息

MA5-16358

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看4篇已发表文章 4篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:20

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

proprietary

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SP31

抗原

Synthetic peptides of human DOG-1 protein (aa 400-500).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.057 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2, with 1% BSA

内含物

0.1% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2537877

产品详细信息

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is gastrointestinal stromal tumor.

靶标信息

DOG-1, the novel marker, is expressed ubiquitously in gastrointestinal stromal tumors irrespective of kit or PDGFR alpha mutation status.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Identification of potential molecular targets for the treatment of cluster 1 human pheochromocytoma and paraganglioma via comprehensive proteomic characterization.

Clinical proteomics

Vit O,Talacko P,Musil Z,Hartmann I,Pacak K,Petrak J

Published figure using DOG-1 recombinant monoclonal antibody (Product # MA5-16358) in Western Blot

Mon Sep 25 00:00:00 EDT 2023

TMEM16A/ANO1 Inhibits Apoptosis Via Downregulation of Bim Expression.

Clinical cancer research : an official journal of the American Association for Cancer Research

Godse NR,Khan N,Yochum ZA,Gomez-Casal R,Kemp C,Shiwarski DJ,Seethala RS,Kulich S,Seshadri M,Burns TF,Duvvuri U

Published figure using DOG-1 recombinant monoclonal antibody (Product # MA5-16358) in Western Blot

Fri Dec 01 00:00:00 EST 2017

TMEM16A/ANO1 is differentially expressed in HPV-negative versus HPV-positive head and neck squamous cell carcinoma through promoter methylation.

Scientific reports

Dixit R,Kemp C,Kulich S,Seethala R,Chiosea S,Ling S,Ha PK,Duvvuri U

MA5-16358 was used in immunohistochemistry to elucidate differential expression of TMEM16A/ANO1 in HPV-positive and HPV-negative head and neck squamous cell carcinoma through promoter methylation

Fri Nov 13 00:00:00 EST 2015

Plexiform fibromyxoma (plexiform angiomyxoid myofibroblastic tumor) of stomach: an unusual presentation as a fistulating abscess.

International journal of surgical pathology

Lee PW,Yau DT,Lau PP,Chan JK

MA5-16358 was used in immunohistochemistry to report on a case of plexiform fibromyxoma of the stomach

Thu May 01 00:00:00 EDT 2014

Na+/Ca2+ exchangers regulate the migration and proliferation of human gastric myofibroblasts.

American journal of physiology. Gastrointestinal and liver physiology

Kemény LV,Schnúr A,Czepán M,Rakonczay Z,Gál E,Lonovics J,Lázár G,Simonka Z,Venglovecz V,Maléth J,Judák L,Németh IB,Szabó K,Almássy J,Virág L,Geisz A,Tiszlavicz L,Yule DI,Wittmann T,Varró A,Hegyi P

MA5-16358 was used in immunohistochemistry to study the mechanisms by which Na(+)/Ca(2+) exchangers modulate human gastric myofibroblast proliferation and migration

Tue Oct 15 00:00:00 EDT 2013

Contribution of DOG1 expression to the diagnosis of gastrointestinal stromal tumors.

Pathology, research and practice

Kara T,Serinsoz E,Arpaci RB,Gubur O,Orekici G,Ata A,Colak T,Arican A

MA5-16358 was used in immunohistochemistry to study the diagnostic value of the immunohistochemical expression of DOG1 in gastrointestinal stromal tumors

Mon Jul 01 00:00:00 EDT 2013

生物信息学

蛋白别名: anoctamin 1, calcium activated chloride channel; Anoctamin-1; Ca2+-activated Cl- channel; calcium activated chloride channel; Discovered on gastrointestinal stromal tumors protein 1; FLJ10261; oral cancer overexpressed 2; Oral cancer overexpressed protein 2; Transmembrane protein 16A; transmembrane protein 16A (eight membrane-spanning domains); Tumor-amplified and overexpressed sequence 2

查看更多收起

基因别名: ANO1; DOG1; ORAOV2; TAOS2; TMEM16A

查看更多收起

UniProt ID: (Human) Q5XXA6

查看更多收起

Entrez Gene ID: (Human) 55107

查看更多收起

功能

calcium activated cation channel activity intracellular calcium activated chloride channel activity voltage-gated chloride channel activity chloride channel activity protein binding iodide transmembrane transporter activity protein dimerization activity

参与通路