Invitrogen

DRD2 Recombinant Rabbit Monoclonal Antibody (HL1584)

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抗体检测数据 (8)

DRD2 Antibody in Immunocytochemistry (ICC/IF)

DRD2 Antibody in Immunocytochemistry (ICC/IF)

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DRD2 Antibody (MA5-47103) in ICC/IF

Immunofluorescent analysis of DRD2 in SH-SY-5Y cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 monoclonal antibody (Product # MA5-47103) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1,000. Blue: Fluoroshield with DAPI.

DRD2 Antibody in Immunocytochemistry (ICC/IF)

DRD2 Antibody in Immunocytochemistry (ICC/IF)

DRD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DRD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DRD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DRD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DRD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DRD2 Antibody in Western Blot (WB)

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DRD2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of DRD2 in SH-SY-5Y cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 monoclonal antibody (Product # MA5-47103) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1,000. Blue: Fluoroshield with DAPI.

DRD2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of DRD2 in DIV9 rat E18 primary cortical neuron cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 monoclonal antibody (Product # MA5-47103) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.

DRD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DRD2 in paraffin-embedded mouse cerebellum. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 monoclonal antibody (Product # MA5-47103) diluted at 1:100. Red: MAP2, a neural marker, stained by MAP2 antibody [GT925] diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

DRD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DRD2 in paraffin-embedded rat cerebellum. Green: Dopamine Receptor D2 stained by Dopamine Receptor D2 monoclonal antibody (Product # MA5-47103) diluted at 1:100. Red: MAP2, a neural marker, stained by MAP2 antibody [GT925] diluted at 1:250. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

DRD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DRD2 in paraffin-embedded cat cerebellum. Samples were incubated in DRD2 Monoclonal antibody (Product # MA5-47103). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

DRD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DRD2 in paraffin-embedded mouse brain. Samples were incubated in DRD2 Monoclonal antibody (Product # MA5-47103). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

DRD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DRD2 in paraffin-embedded mouse brain. Samples were incubated in DRD2 Monoclonal antibody (Product # MA5-47103). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

DRD2 Antibody in Western Blot (WB) — Western blot analysis of DRD2 in Various tissue extracts (50 µg) separated by 10% SDS-PAGE. Membrane was blotted with DRD2 Monoclonal antibody (Product # MA5-47103) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.

产品信息

MA5-47103

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

-

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

-

免疫细胞化学 (ICC/IF)

Assay-dependent

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

HL1584

抗原

Recombinant protein encompassing a sequence within the N-terminus region of human Dopamine Receptor D2. The exact sequence is proprietary.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2938175

产品详细信息

Store as concentrated solution.

Centrifuge briefly prior to opening vial.

靶标信息

The DRD2 Dopamine Receptor is involved in the neuromodulation of appetitive behaviors. DRD2 may be linked to alcoholism and other substance-use disorders. Mutations in DRD2 in mice lead to the suppression of reward behavior with morphine and to the reduction of spontaneous movements, a phenotype resembling Parkinsons disease. Three alternatively spliced isoforms have been identified. DRD2 has been reported to be expressed in various regions of the brain, as well as in aorta, artery, eye, and spinal cord. ESTs have been isolated from normal pituitary and in brain, colon, lung, pancreas, and skeletal muscle cancer libraries.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: D(2) dopamine receptor; D2 receptor; Dopamine 2 receptor; Dopamine D2 receptor; dopamine receptor 2; dopamine receptor D2 isoform; seven transmembrane helix receptor

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基因别名: D2DR; D2R; Drd-2; DRD2

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UniProt ID: (Human) P14416, (Mouse) P61168, (Rat) P61169

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Entrez Gene ID: (Human) 1813, (Mouse) 13489, (Rat) 24318

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dopamine neurotransmitter receptor activity, coupled via Gi/Go adrenergic receptor activity protein binding drug binding dopamine binding ionotropic glutamate receptor binding identical protein binding protein homodimerization activity protein heterodimerization activity signal transducer activity G-protein coupled receptor activity dopamine neurotransmitter receptor activity receptor binding G-protein coupled receptor

参与通路

temperature homeostasis response to hypoxia synaptic transmission, dopaminergic response to amphetamine neurological system process involved in regulation of systemic arterial blood pressure regulation of heart rate regulation of sodium ion transport G-protein coupled receptor internalization positive regulation of neuroblast proliferation positive regulation of receptor internalization negative regulation of adenylate cyclase activity adenylate cyclase-inhibiting dopamine receptor signaling pathway neuron-neuron synaptic transmission axonogenesis synapse assembly sensory perception of smell long-term memory grooming behavior locomotory behavior adult walking behavior feeding behavior protein localization negative regulation of cell proliferation associative learning visual learning response to light stimulus response to toxic substance response to iron ion regulation of dopamine secretion response to inactivity Wnt signaling pathway striatum development orbitofrontal cortex development cerebral cortex GABAergic interneuron migration adenohypophysis development negative regulation of cell migration peristalsis regulation of cAMP metabolic process auditory behavior activation of protein kinase activity regulation of synaptic transmission, GABAergic positive regulation of cytokinesis circadian regulation of gene expression negative regulation of dopamine secretion response to histamine response to nicotine positive regulation of urine volume positive regulation of renal sodium excretion positive regulation of multicellular organism growth response to cocaine negative regulation of circadian sleep/wake cycle, sleep dopamine metabolic process response to drug regulation of potassium ion transport response to morphine pigmentation regulation of phosphoprotein phosphatase activity positive regulation of G-protein coupled receptor protein signaling pathway negative regulation of blood pressure negative regulation of innate immune response positive regulation of transcription from RNA polymerase II promoter phosphatidylinositol metabolic process negative regulation of insulin secretion acid secretion behavioral response to cocaine behavioral response to ethanol regulation of long-term neuronal synaptic plasticity response to axon injury branching morphogenesis of a nerve arachidonic acid secretion negative regulation of protein secretion release of sequestered calcium ion into cytosol negative regulation of cytosolic calcium ion concentration regulation of dopamine uptake involved in synaptic transmission positive regulation of dopamine uptake involved in synaptic transmission regulation of synapse structural plasticity negative regulation of protein kinase B signaling negative regulation of synaptic transmission, glutamatergic positive regulation of growth hormone secretion prepulse inhibition phospholipase C-activating dopamine receptor signaling pathway negative regulation of dopamine receptor signaling pathway positive regulation of ERK1 and ERK2 cascade adenylate cyclase-activating adrenergic receptor signaling pathway regulation of locomotion involved in locomotory behavior chemical synaptic transmission, postsynaptic positive regulation of glial cell-derived neurotrophic factor secretion positive regulation of long-term synaptic potentiation negative regulation of voltage-gated calcium channel activity startle response signal transduction G-protein coupled receptor signaling pathway adenylate cyclase-modulating G-protein coupled receptor signaling pathway dopamine receptor signaling pathway chemical synaptic transmission adult behavior forebrain development intracellular signal transduction regulation of MAPK cascade modulation of synaptic transmission positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway cellular potassium ion homeostasis response to ethanol positive regulation of mitotic nuclear division