Invitrogen

DTYMK Recombinant Rabbit Monoclonal Antibody (23GB5235)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他19个DTYMK抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

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DTYMK Antibody (MA5-53870) in WB

Western Blot analysis of DTYMK. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DTYMK recombinant monoclonal antibody (Product # MA5-53870) with a dilution of 1:5,000, followed by HRP-conjugated goat anti-rabbit secondary antibody with a dilution of 1:20,000. Image was developed using FeQTM ECL Substrate Kit.

Product Image Gallery

DTYMK Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with DTYMK shRNA and decrease in signal intensity was observed in Western Blotting using Anti-DTYMK Recombinant Rabbit Monoclonal Antibody (23GB5235) (Product # MA5-53870) at a dilution of 1:5,000. Secondary antibody used was a goat anti-rabbit (HRP) at a dilution of 1:20,000. {KD}

产品信息

MA5-53870

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

流式细胞分析 (Flow)

1:2,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293F

分类

Recombinant Monoclonal

类型

Antibody

克隆号

23GB5235

抗原

A synthesized peptide derived from human DTYMK (160-200AA).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice (domestic); Dry ice (international)

靶标信息

Thymidylate kinase (DTYMK) catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis. DTYMK is essential for DNA synthesis and is an important intermediate enzyme in the pathway of many pyrimidine analog drugs, including 5-Fluorouracil (5-FU), the chemotherapeutic drug of choice for several solid tumors. The human DTYMK gene was isolated via functional complementation of a Saccharomyces cerevisiae cell cycle mutant, cdc8. DTYMK mRNA levels and enzyme activities are regulated at the level of cell cycle and cell growth pathways.

仅用于科研。不用于诊断过程。未经明确授权不得转售。