Product References
A fetal tumor suppressor axis abrogates MLL-fusion-driven acute myeloid leukemia.
Cell reports
Eldeeb M,Yuan O,Guzzi N,Thi Ngoc PC,Konturek-Ciesla A,Kristiansen TA,Muthukumar S,Magee J,Bellodi C,Yuan J,Bryder D
PA1-984B was used in Western Blotting to investigate the impact of LIN28B on AML using a dual-transgenic mouse model that co-expresses MLL-ENL and LIN28B.
Tue Feb 28 00:00:00 EST 2023
Gcap14 is a microtubule plus-end-tracking protein coordinating microtubule-actin crosstalk during neurodevelopment.
Proceedings of the National Academy of Sciences of the United States of America
Mun DJ,Goo BS,Suh BK,Hong JH,Woo Y,Kim SJ,Kim S,Lee SB,Won Y,Yoo JY,Cho E,Jang EJ,Nhung TTM,Triet HM,An H,Lee H,Nguyen MD,Park SY,Baek ST,Park SK
PA1-984B was used in Western blotting following immunoprecipitation to propose that the Gcap14-Ndel1 complex is fundamental for cytoskeletal remodeling during neurodevelopmental processes such as neuronal processes elongation and neuronal migration.
Tue Feb 21 00:00:00 EST 2023
Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells.
STAR protocols
Calle B,Gonzalez-Rodriguez E,Mahoney KE,Cioce A,Bineva-Todd G,Tastan OY,Roustan C,Flynn H,Malaker SA,Schumann B
PA1-984B was used in Immunocytochemistry-immunofluorescence to describe a protocol to develop chemical, bioorthogonal reporters for the activity of the GalNAc-T family of glycosyltransferases using a tactic termed bump-and-hole engineering.
Fri Mar 17 00:00:00 EDT 2023
INPP5E regulates CD3ζ enrichment at the immune synapse by phosphoinositide distribution control.
Communications biology
Chiu TY,Lo CH,Lin YH,Lai YD,Lin SS,Fang YT,Huang WS,Huang SY,Tsai PY,Yang FH,Chong WM,Wu YC,Tsai HC,Liu YW,Hsu CL,Liao JC,Wang WJ
PA1-984B was used in Western Blotting to suggest that INPP5E is a new player in phosphoinositide manipulation at the synapse, controlling the TCR signaling cascade.
Tue Sep 05 00:00:00 EDT 2023
CD-NTase family member MB21D2 promotes cGAS-mediated antiviral and antitumor immunity.
Cell death and differentiation
Liu H,Yan Z,Zhu D,Xu H,Liu F,Chen T,Zhang H,Zheng Y,Liu B,Zhang L,Zhao W,Gao C
PA1-984B was used in Western Blotting to unveil that the CD-NTase family member MB21D2 contributes to host antiviral and antitumor responses by enhancing cGAS activation.
Sat Apr 01 00:00:00 EDT 2023
VCP/p97 cofactor UBXN1/SAKS1 regulates mitophagy by modulating MFN2 removal from mitochondria.
Autophagy
Mengus C,Neutzner M,Bento ACPF,Bippes CC,Kohler C,Decembrini S,Häusel J,Hemion C,Sironi L,Frank S,Scholl HPN,Neutzner A
PA1-984B was used in Immunocytochemistry-immunofluorescence to connect the VCP cofactor UBXN1 to the initiation and maintenance phase of PRKN-dependent mitophagy, and indicate that, upon mitochondrial stress induction, MFN2 removal from mitochondria occurs through a specialized process.
Sat Jan 01 00:00:00 EST 2022
Non-invasive administration of AAV to target lung parenchymal cells and develop SARS-CoV-2-susceptible mice.
Molecular therapy : the journal of the American Society of Gene Therapy
Yang MS,Park MJ,Lee J,Oh B,Kang KW,Kim Y,Lee SM,Lim JO,Jung TY,Park JH,Park SC,Lim YS,Hwang SB,Lyoo KS,Kim DI,Kim B
PA1-984B was used in Western Blot to demonstrate the ease and rapidness of the intratracheal administration of AAV for targeting lung tissue in mice, and this can be used to study diverse pulmonary diseases.
Wed May 04 00:00:00 EDT 2022
A loss-of-function cysteine mutant in fibulin-3 (EFEMP1) forms aberrant extracellular disulfide-linked homodimers and alters extracellular matrix composition.
Human mutation
Woodard DR,Daniel S,Nakahara E,Abbas A,DiCesare SM,Collier GE,Hulleman JD
PA1-984B was used in Western Blotting to characterize how one of these mutations, c.163T>C; p.Cys55Arg (C55R), disrupts F3 secretion, quaternary structure, and function by forming unique extracellular disulfide-linked homodimers.
Thu Dec 01 00:00:00 EST 2022
Disentangling autoproteolytic cleavage from tethered agonist-dependent activation of the adhesion receptor ADGRL3.
The Journal of biological chemistry
Perry-Hauser NA,VanDyck MW,Lee KH,Shi L,Javitch JA
PA1-984B was used in Western Blotting to present a construct that disrupts tethered agonism while retaining autoproteolytic cleavage, providing a tool to disentangle these functions in vivo.
Thu Dec 01 00:00:00 EST 2022
Circadian REV-ERBs repress E4bp4 to activate NAMPT-dependent NAD+ biosynthesis and sustain cardiac function.
Nature cardiovascular research
Dierickx P,Zhu K,Carpenter BJ,Jiang C,Vermunt MW,Xiao Y,Luongo TS,Yamamoto T,Martí-Pàmies Í,Mia S,Latimer M,Diwan A,Zhao J,Hauck AK,Krusen B,Nguyen HCB,Blobel GA,Kelly DP,Pei L,Baur JA,Young ME,Lazar MA
PA1-984B was used in Western Blot to investigate the role of the nuclear receptors REV-ERBα and β in the heart by mice with cardiomyocyte (CM)-specific deletion of both Rev-erbs.
Sat Jan 01 00:00:00 EST 2022
Cleavage of mitochondrial homeostasis regulator PGAM5 by the intramembrane protease PARL is governed by transmembrane helix dynamics and oligomeric state.
The Journal of biological chemistry
Siebert V,Silber M,Heuten E,Muhle-Goll C,Lemberg MK
PA1-984B was used in Immunocytochemistry-immunoflourescence to investigate if several polar transmembrane residues in PGAM5 distant from the cleavage site serve as key determinants for its PARL-catalyzed cleavage.
Thu Sep 01 00:00:00 EDT 2022
A specialized Hsp90 co-chaperone network regulates steroid hormone receptor response to ligand.
Cell reports
Backe SJ,Sager RA,Regan BR,Sit J,Major LA,Bratslavsky G,Woodford MR,Bourboulia D,Mollapour M
PA1-984B was used in Immunocytochemistry, Western Blot, Immunoprecipitation to provide a model for the responsiveness of the steroid hormone receptor activation upon ligand binding as a consequence of their association with specific Hsp90:co-chaperone subpopulations.
Tue Jul 12 00:00:00 EDT 2022
A Proximity biotinylation assay with a host protein bait reveals multiple factors modulating enterovirus replication.
PLoS pathogens
Moghimi S,Viktorova EG,Gabaglio S,Zimina A,Budnik B,Wynn BG,Sztul E,Belov GA
PA1-984B was used in Western Blotting to investigate the proteins recruited to the replication organelles of poliovirus, a representative of the genus Enterovirus of the Picornaviridae family.
Sat Oct 01 00:00:00 EDT 2022
Cardiac troponin T and autoimmunity in skeletal muscle aging.
GeroScience
Zhang T,Feng X,Dong J,Xu Z,Feng B,Haas KM,Cawthon PM,Beavers KM,Nicklas B,Kritchevsky S
PA1-984B was used in Western Blotting to suggest a novel cTnT-mediated autoimmune response may be an indicator of sarcopenia.
Mon Aug 01 00:00:00 EDT 2022
Nuclear receptor HR96 up-regulates cytochrome P450 for insecticide detoxification in Tribolium castaneum.
Pest management science
Kim IY,Choi B,Park WR,Kim YJ,Kim BE,Mun S,Choi HS,Kim DK
PA1-984B was used in Western Blotting to demonstrate that TcHR96 plays a major role in transcriptional control of P450 enzyme for imidacloprid detoxification, controlling which might facilitate the regulation of insecticide tolerance in T. castaneum.
Sat Jan 01 00:00:00 EST 2022
A Yeast BiFC-seq Method for Genome-wide Interactome Mapping.
Genomics, proteomics & bioinformatics
Shang L,Zhang Y,Liu Y,Jin C,Yuan Y,Tian C,Ni M,Bo X,Zhang L,Li D,He F,Wang J
PA1-984B was used in Immunocytochemistry, Western Blot, ChIP Assay to show that BiFC-seq is a highly sensitive, rapid, and economical method for genome-wide interactome mapping.
Mon Aug 01 00:00:00 EDT 2022
USP5 attenuates NLRP3 inflammasome activation by promoting autophagic degradation of NLRP3.
Autophagy
Cai B,Zhao J,Zhang Y,Liu Y,Ma C,Yi F,Zheng Y,Zhang L,Chen T,Liu H,Liu B,Gao C
PA1-984B was used in Western Blot to demonstrate that USP5 is a key scaffold protein recruiting the E3 ligase MARCHF7 to NLRP3, and promoting autophagic degradation of NLRP3.
Sun May 01 00:00:00 EDT 2022
O-GlcNAcylation suppresses TRAP1 activity and promotes mitochondrial respiration.
Cell stress & chaperones
Kim S,Backe SJ,Wengert LA,Johnson AE,Isakov RV,Bratslavsky MS,Woodford MR
PA1-984B was used in Proximity Ligation Assay (PLA) to demonstrate that TRAP1-O-GlcNAcylation decreases TRAP1 ATPase activity, leading to increased mitochondrial metabolism.
Thu Sep 01 00:00:00 EDT 2022
CircEIF3H-IGF2BP2-HuR scaffold complex promotes TNBC progression via stabilizing HSPD1/RBM8A/G3BP1 mRNA.
Cell death discovery
Song X,Chen B,Liang Y,Li Y,Zhang H,Han D,Wang Y,Ye F,Wang L,Zhao W,Yang Q
PA1-984B was used in Western Blot, Immunoprecipitation to provide insight into a novel circRNA, circEIF3H, with significant cancer-promoting function via serving as a scaffold for IGF2BP2/HuR.
Sat May 14 00:00:00 EDT 2022
CircEIF3H-IGF2BP2-HuR scaffold complex promotes TNBC progression via stabilizing HSPD1/RBM8A/G3BP1 mRNA.
Cell death discovery
Song X,Chen B,Liang Y,Li Y,Zhang H,Han D,Wang Y,Ye F,Wang L,Zhao W,Yang Q
PA1-984B was used in Western Blot, Immunoprecipitation to provide insight into a novel circRNA, circEIF3H, with significant cancer-promoting function via serving as a scaffold for IGF2BP2/HuR.
Sat May 14 00:00:00 EDT 2022
A somatic mutation in moesin drives progression into acute myeloid leukemia.
Science advances
Yuan O,Ugale A,de Marchi T,Anthonydhason V,Konturek-Ciesla A,Wan H,Eldeeb M,Drabe C,Jassinskaja M,Hansson J,Hidalgo I,Velasco-Hernandez T,Cammenga J,Magee JA,Niméus E,Bryder D
PA1-984B was used in Western Blotting to demonstrate a critical role of ERM proteins in AML, with implications also for human cancer.
Fri Apr 22 00:00:00 EDT 2022
Ribosome ADP-ribosylation inhibits translation and maintains proteostasis in cancers.
Cell
Challa S,Khulpateea BR,Nandu T,Camacho CV,Ryu KW,Chen H,Peng Y,Lea JS,Kraus WL
PA1-984B was used in Western Blot, Immunocytochemistry to demonstrate that ribosome MARylation promotes protein homeostasis in cancers by fine-tuning the levels of protein synthesis and preventing toxic protein aggregation.
Thu Aug 19 00:00:00 EDT 2021
Ribosome ADP-ribosylation inhibits translation and maintains proteostasis in cancers.
Cell
Challa S,Khulpateea BR,Nandu T,Camacho CV,Ryu KW,Chen H,Peng Y,Lea JS,Kraus WL
PA1-984B was used in Western Blot, Immunocytochemistry to demonstrate that ribosome MARylation promotes protein homeostasis in cancers by fine-tuning the levels of protein synthesis and preventing toxic protein aggregation.
Thu Aug 19 00:00:00 EDT 2021
The Lipogenic Regulator SREBP2 Induces Transferrin in Circulating Melanoma Cells and Suppresses Ferroptosis.
Cancer discovery
Hong X,Roh W,Sullivan RJ,Wong KHK,Wittner BS,Guo H,Dubash TD,Sade-Feldman M,Wesley B,Horwitz E,Boland GM,Marvin DL,Bonesteel T,Lu C,Aguet F,Burr R,Freeman SS,Parida L,Calhoun K,Jewett MK,Nieman LT,Hacohen N,Näär AM,Ting DT,Toner M,Stott SL,Getz G,Maheswaran S,Haber DA
PA1-984B was used in Western Blotting to show that single CTCs from melanoma patients coordinately upregulate lipogenesis and iron homeostasis pathways.
Mon Mar 01 00:00:00 EST 2021
Wnt signaling recruits KIF2A to the spindle to ensure chromosome congression and alignment during mitosis.
Proceedings of the National Academy of Sciences of the United States of America
Bufe A,García Del Arco A,Hennecke M,de Jaime-Soguero A,Ostermaier M,Lin YC,Ciprianidis A,Hattemer J,Engel U,Beli P,Bastians H,Acebrón SP
PA1-984B was used in Immunocytochemistry to show Wnt signaling monitors KIF2A activity at the spindle poles during mitosis.
Tue Aug 24 00:00:00 EDT 2021
OTUD1 Regulates Antifungal Innate Immunity through Deubiquitination of CARD9.
Journal of immunology (Baltimore, Md. : 1950)
Chen X,Zhang H,Wang X,Shao Z,Li Y,Zhao G,Liu F,Liu B,Zheng Y,Chen T,Zheng H,Zhang L,Gao C
PA1-984B was used in Functional assay to understand the role of ovarian tumour deubiquitinase 1 as a regulator of CARD9, with the use of transgenic mice.
Thu Apr 15 00:00:00 EDT 2021
TRIM21 inhibits porcine epidemic diarrhea virus proliferation by proteasomal degradation of the nucleocapsid protein.
Archives of virology
Wang H,Chen X,Kong N,Jiao Y,Sun D,Dong S,Qin W,Zhai H,Yu L,Zheng H,Tong W,Yu H,Tong G,Shan T
PA1-984B was used in Western Blot to provide information about the role of TRIM21 in PEDV proliferation and increase our understanding of host-virus interactions.
Thu Jul 01 00:00:00 EDT 2021
Disrupted-in-schizophrenia 1 enhances the quality of circadian rhythm by stabilizing BMAL1.
Translational psychiatry
Lee SB,Park J,Kwak Y,Park YU,Nhung TTM,Suh BK,Woo Y,Suh Y,Cho E,Cho S,Park SK
PA1-984B was used in Immunoprecipitation to demonstrate that the oscillation of DISC1 expression is under the control of CLOCK and BMAL1, and that DISC1 contributes to the core circadian system by regulating BMAL1 stability.
Thu Feb 04 00:00:00 EST 2021
Poly(C)-binding Protein 2 Regulates the p53 Expression via Interactions with the 5'-Terminal Region of p53 mRNA.
International journal of molecular sciences
Janecki DM,Swiatkowska A,Szpotkowska J,Urbanowicz A,Kabacińska M,Szpotkowski K,Ciesiołka J
PA1-984B was used in Western Blotting to show that under stress conditions PCBP2 also modulates p53 translation through binding to the 5' terminus of p53 mRNA.
Fri Dec 10 00:00:00 EST 2021
Cloning of a Passage-Free SARS-CoV-2 Genome and Mutagenesis Using Red Recombination.
International journal of molecular sciences
Herrmann A,Jungnickl D,Cordsmeier A,Peter AS,Überla K,Ensser A
PA1-984B was used in Western Blot, Immunocytochemistry to describe the assembly of a passage-free SARS-CoV-2 B.1 genome into a single bacterial artificial chromosome and its manipulation by Lambda Red-based recombination, and show that this reverse genetics system can be easily used to recover wild-type and mutant viruses from transfected cells.
Wed Sep 22 00:00:00 EDT 2021
Cloning of a Passage-Free SARS-CoV-2 Genome and Mutagenesis Using Red Recombination.
International journal of molecular sciences
Herrmann A,Jungnickl D,Cordsmeier A,Peter AS,Überla K,Ensser A
PA1-984B was used in Western Blot, Immunocytochemistry to describe the assembly of a passage-free SARS-CoV-2 B.1 genome into a single bacterial artificial chromosome and its manipulation by Lambda Red-based recombination, and show that this reverse genetics system can be easily used to recover wild-type and mutant viruses from transfected cells.
Wed Sep 22 00:00:00 EDT 2021
TrkA mediates effect of novel KIDINS220 mutation in human brain ventriculomegaly.
Human molecular genetics
Jacquemin V,Antoine M,Duerinckx S,Massart A,Desir J,Perazzolo C,Cassart M,Thomas D,Segers V,Lecomte S,Abramowicz M,Pirson I
PA1-984B was used in Western Blotting to demonstrate that the binding of KIDINS220 to TrkA is diminished by the deletion mutation.
Thu Feb 04 00:00:00 EST 2021
The middle lipin domain adopts a membrane-binding dimeric protein fold.
Nature communications
Gu W,Gao S,Wang H,Fleming KD,Hoffmann RM,Yang JW,Patel NM,Choi YM,Burke JE,Reue K,Airola MV
PA1-984B was used in Western Blotting to demonstrate that mechanical forces shift fibroblasts toward pro-fibrotic phenotypes driven by ERK-YAP activation, leading to myofibroblast differentiation and excessive collagen production.
Thu Aug 05 00:00:00 EDT 2021
The folate cycle enzyme MTHFD2 induces cancer immune evasion through PD-L1 up-regulation.
Nature communications
Shang M,Yang H,Yang R,Chen T,Fu Y,Li Y,Fang X,Zhang K,Zhang J,Li H,Cao X,Gu J,Xiao J,Zhang Q,Liu X,Yu Q,Wang T
PA1-984B was used in Western Blot to functionally screen metabolic genes that rescue tumour cells from effector T cell cytotoxicity, and identify the embryo- and tumour-specific folate cycle enzyme methylenetetrahydrofolate dehydrogenase 2 (MTHFD2).
Mon Mar 29 00:00:00 EDT 2021
Formation of Non-Nucleoplasmic Proteasome Foci during the Late Stage of Hyperosmotic Stress.
Cells
Lee J,Le LTHL,Kim E,Lee MJ
PA1-984B was used in Western Blot to identify the biochemical and pathophysiological features of reverse compartmentalisation for the pro-survival mechanisms of protein misfolding cellular stress.
Tue Sep 21 00:00:00 EDT 2021
Effects of Hydrogen Peroxide Stress on the Nucleolar Redox Environment and Pre-rRNA Maturation.
Frontiers in molecular biosciences
Sapio RT,Burns CJ,Pestov DG
PA1-984B was used in Immunocytochemistry to introduce new tools for studies of the redox biology of the mammalian nucleolus and identifies pre-rRNA maturation steps sensitive to H2O2 stress.
Mon May 17 00:00:00 EDT 2021
The DNA-binding protein CST associates with the cohesin complex and promotes chromosome cohesion.
The Journal of biological chemistry
Schuck PL,Ball LE,Stewart JA
PA1-984B was used in Immunocytochemistry, Western Blot to propose that CST aids in the maintenance of SCC at stalled replication forks to prevent premature cohesion loss.
Wed Sep 01 00:00:00 EDT 2021
Proteomic Identification of Coxiella burnetii Effector Proteins Targeted to the Host Cell Mitochondria During Infection.
Molecular & cellular proteomics : MCP
Fielden LF,Scott NE,Palmer CS,Khoo CA,Newton HJ,Stojanovski D
PA1-984B was used in Western Blotting to develop a novel strategy to examine effector proteins in the context of infection.
Mon Mar 14 00:00:00 EDT 2022
SOD1 regulates ribosome biogenesis in KRAS mutant non-small cell lung cancer.
Nature communications
Wang X,Zhang H,Sapio R,Yang J,Wong J,Zhang X,Guo JY,Pine S,Van Remmen H,Li H,White E,Liu C,Kiledjian M,Pestov DG,Steven Zheng XF
PA1-984B was used in Proximity Ligation Assay to describe the generation of an inducible Sod1 knockout in KRAS-driven NSCLC mouse model.
Thu Apr 15 00:00:00 EDT 2021
mRNA localization mediates maturation of cytoplasmic cilia in Drosophila spermatogenesis.
The Journal of cell biology
Fingerhut JM,Yamashita YM
PA1-984B was used in Immunocytochemistry to show that a novel RNP granule containing the mRNAs for axonemal dynein motor proteins becomes highly polarized to the distal end of the cilia during cytoplasmic ciliogenesis in Drosophila sperm.
Mon Sep 07 00:00:00 EDT 2020
Stress-induced phosphorylation of CLIP-170 by JNK promotes microtubule rescue.
The Journal of cell biology
Henrie H,Bakhos-Douaihy D,Cantaloube I,Pilon A,Talantikite M,Stoppin-Mellet V,Baillet A,Poüs C,Benoit B
PA1-984B was used in Western Blotting to show the effect if CLIP-170 phosphorylation by JNK on microtubule dynamics.
Mon Jul 06 00:00:00 EDT 2020
Arhgef5 Binds α-Dystrobrevin 1 and Regulates Neuromuscular Junction Integrity.
Frontiers in molecular neuroscience
Bernadzki KM,Daszczuk P,Rojek KO,Pęziński M,Gawor M,Pradhan BS,de Cicco T,Bijata M,Bijata K,Włodarczyk J,Prószyński TJ,Niewiadomski P
PA1-984B was used in Immunoprecipitation to identify novel proteins that are important for NMJ organization.
Mon Sep 28 00:00:00 EDT 2020
The PP1 regulator PPP1R2 coordinately regulates AURKA and PP1 to control centrosome phosphorylation and maintain central spindle architecture.
BMC molecular and cell biology
Bresch AM,Yerich N,Wang R,Sperry AO
PA1-984B was used in Immunocytochemistry-immunofluorescence to reveal the involvement of protein phosphatase 1 regulatory subunit 2 in coordinating protein phosphatase 1 and AURKA activity during cytokinesis.
Wed Nov 25 00:00:00 EST 2020
Phosphorylation of Connexin36 near the C-terminus switches binding affinities for PDZ-domain and 14-3-3 proteins in vitro.
Scientific reports
Tetenborg S,Wang HY,Nemitz L,Depping A,Espejo AB,Aseervatham J,Bedford MT,Janssen-Bienhold U,O'Brien J,Dedek K
PA1-984B was used in Immunocytochemistry-immunoflourescence to reveal that phosphorylation sites may serve as a molecular switch and influence the affinity of the PDZ binding domain for its binding partners.
Tue Oct 27 00:00:00 EDT 2020
Human Cytomegalovirus Genomes Survive Mitosis via the IE19 Chromatin-Tethering Domain.
mBio
Lyon SM,Yetming KD,Paulus C,Nevels M,Kalejta RF
PA1-984B was used in Western Blot, ChIP assay to report that the human cytomegalovirus (HCMV) genome is maintained during mitosis by the CTD of the viral IE19 protein.
Tue Sep 29 00:00:00 EDT 2020
Human Cytomegalovirus Genomes Survive Mitosis via the IE19 Chromatin-Tethering Domain.
mBio
Lyon SM,Yetming KD,Paulus C,Nevels M,Kalejta RF
PA1-984B was used in Western Blot, ChIP assay to report that the human cytomegalovirus (HCMV) genome is maintained during mitosis by the CTD of the viral IE19 protein.
Tue Sep 29 00:00:00 EDT 2020
A Ca2+-ATPase Regulates E-cadherin Biogenesis and Epithelial-Mesenchymal Transition in Breast Cancer Cells.
Molecular cancer research : MCR
Dang DK,Makena MR,Llongueras JP,Prasad H,Ko M,Bandral M,Rao R
PA1-984B was used in Flow cytometry/Cell sorting to investigate the posttranslational control of E-cadherin and downstream Hippo signalling in breast cancer cells.
Thu Aug 01 00:00:00 EDT 2019
Exploiting Prophage-Mediated Lysis for Biotherapeutic Release by Lactobacillus reuteri.
Applied and environmental microbiology
Alexander LM,Oh JH,Stapleton DS,Schueler KL,Keller MP,Attie AD,van Pijkeren JP
PA1-984B was used in Western Blotting to demonstrate that an intracellularly accumulated recombinant protein is released following prophage activation.
Wed May 15 00:00:00 EDT 2019
An efficient and quantitative assay for epitope-tagged therapeutic protein development with a capillary western system.
Bioanalysis
Li Y,Duah E,Long N,Persaud A,VanGundy Z,Magliery T,Poi MJ
PA1-984B was used in Simple Western - Size to develop and validate a reliable, robust and efficient assay to detect and quantify biologic compounds in vitro and in vivo during early stage of a biotherapeutic agent discovery.
Fri Mar 01 00:00:00 EST 2019
Lzts1 controls both neuronal delamination and outer radial glial-like cell generation during mammalian cerebral development.
Nature communications
Kawaue T,Shitamukai A,Nagasaka A,Tsunekawa Y,Shinoda T,Saito K,Terada R,Bilgic M,Miyata T,Matsuzaki F,Kawaguchi A
PA1-984B was used in Immunohistochemistry to study the mechanisms underlying neuronal delamination and outer glial like cell regeneration.
Tue Jun 25 00:00:00 EDT 2019
Proteomic Phosphosite Analysis Identified Crucial NPM-ALK-Mediated NIPA Serine and Threonine Residues.
International journal of molecular sciences
Gengenbacher A,Müller-Rudorf A,Poggio T,Gräßel L,Dumit VI,Kreutmair S,Lippert LJ,Duyster J,Illert AL
PA1-984B was used in Western blotting to elucidate the importance of NIPA phosphorylation by NPM-ALK for the interaction of the two proteins and proliferation potential of respective cells: Silencing of the five crucial NIPA serine/threonine residues led to a highly enhanced NIPA-NPM-ALK binding capacity as well as a slightly reduced proliferation in Ba/F3 cells.
Tue Aug 20 00:00:00 EDT 2019
USP7 Regulates Cytokinesis through FBXO38 and KIF20B.
Scientific reports
Georges A,Coyaud E,Marcon E,Greenblatt J,Raught B,Frappier L
PA1-984B was used in Western Blotting to reveal a novel interaction of ubiquitin specific protease 7 with FBXO38.
Mon Feb 25 00:00:00 EST 2019
Truncated BRPF1 Cooperates with Smoothened to Promote Adult Shh Medulloblastoma.
Cell reports
Aiello G,Ballabio C,Ruggeri R,Fagnocchi L,Anderle M,Morassut I,Caron D,Garilli F,Gianno F,Giangaspero F,Piazza S,Romanel A,Zippo A,Tiberi L
PA1-984B was used in Immunohistochemistry to investigate if Shh pathway activation through SmoM2 expression promotes postmitotic neurons dedifferentiation.
Tue Dec 17 00:00:00 EST 2019
Isocytosine deaminase Vcz as a novel tool for the prodrug cancer therapy.
BMC cancer
Kazlauskas A,Darinskas A,Meškys R,Tamašauskas A,Urbonavičius J
PA1-984B was used in Western Blotting to test the applicability of the isocysteine deaminase Vcz/5-fluoroisocytosine pair as a potential suicide gene therapy tool.
Mon Mar 04 00:00:00 EST 2019
Mutation of the co-chaperone Tsc1 in bladder cancer diminishes Hsp90 acetylation and reduces drug sensitivity and selectivity.
Oncotarget
Woodford MR,Hughes M,Sager RA,Backe SJ,Baker-Williams AJ,Bratslavsky MS,Jacob JM,Shapiro O,Wong M,Bratslavsky G,Bourboulia D,Mollapour M
PA1-984B was used in Western blotting following immunoprecipitation to show loss of TSC1 leads to hypoacetylation of Hsp90-K407/K419 and subsequent decreased binding to the Hsp90 inhibitor ganetespib.
Tue Oct 08 00:00:00 EDT 2019
Designing yeast as plant-like hyperaccumulators for heavy metals.
Nature communications
Sun GL,Reynolds EE,Belcher AM
PA1-984B was used in Immunocytochemistry-immunoflourescence to present a systematic approach for transforming yeast into metal hyperaccumulators that are as effective as their plant counterparts.
Fri Nov 08 00:00:00 EST 2019
Human CST suppresses origin licensing and promotes AND-1/Ctf4 chromatin association.
Life science alliance
Wang Y,Brady KS,Caiello BP,Ackerson SM,Stewart JA
PA1-984B was used in Western Blotting to determine the role of human CTC1-STN1-TEN1 alters origin licensing and activation.
Mon Apr 01 00:00:00 EDT 2019
RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination.
Molecular cell
Ribeiro de Almeida C,Dhir S,Dhir A,Moghaddam AE,Sattentau Q,Meinhart A,Proudfoot NJ
PA1-984B was used in Immunoprecipitation to provide evidence for how S-region transcripts interconvert between G4 and R-loop structures to promote IgH class switch recombination.
Thu May 17 00:00:00 EDT 2018
RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination.
Molecular cell
Ribeiro de Almeida C,Dhir S,Dhir A,Moghaddam AE,Sattentau Q,Meinhart A,Proudfoot NJ
PA1-984B was used in Immunoprecipitation to provide evidence for how S-region transcripts interconvert between G4 and R-loop structures to promote IgH class switch recombination.
Thu May 17 00:00:00 EDT 2018
Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators.
The EMBO journal
Robeson AC,Lindblom KR,Wojton J,Kornbluth S,Matsuura K
PA1-984B was used in Immunoprecipitation to isolate study the active caspase-2 dimer and its interactome.
Fri Jul 13 00:00:00 EDT 2018
Dimer-specific immunoprecipitation of active caspase-2 identifies TRAF proteins as novel activators.
The EMBO journal
Robeson AC,Lindblom KR,Wojton J,Kornbluth S,Matsuura K
PA1-984B was used in Immunoprecipitation to isolate study the active caspase-2 dimer and its interactome.
Fri Jul 13 00:00:00 EDT 2018
Arabidopsis pollen tube germination and growth depend on the mitochondrial calcium uniporter complex.
The New phytologist
Selles B,Michaud C,Xiong TC,Leblanc O,Ingouff M
PA1-984B was used in Western Blotting to show that mitochondrial Ca2+ controlled by MCUs is an additional player in Arabidopsis pollen tube germination and growth.
Sun Jul 01 00:00:00 EDT 2018
GRWD1 regulates ribosomal protein L23 levels via the ubiquitin-proteasome system.
Journal of cell science
Watanabe S,Fujiyama H,Takafuji T,Kayama K,Matsumoto M,Nakayama KI,Yoshida K,Sugimoto N,Fujita M
PA1-984B was used in Western Blotting to study the role of Glutamate-rich WD40 repeat-containing 1-induced ribosomal protein L23 proteolysis in the downregulation of p53 and tumourigenesis.
Fri Aug 03 00:00:00 EDT 2018
Detection and Analysis of Extracellular Hsp90 (eHsp90).
Methods in molecular biology (Clifton, N.J.)
Cortes S,Baker-Williams AJ,Mollapour M,Bourboulia D
PA1-984B was used in Western Blotting to study Heat shock protein 90 (Hsp90) on stabilization functions and interactions with extracellular clients.
Fri Jul 27 00:00:00 EDT 2018
The RNA-editing enzyme ADAR promotes lung adenocarcinoma migration and invasion by stabilizing FAK.
Science signaling
Amin EM,Liu Y,Deng S,Tan KS,Chudgar N,Mayo MW,Sanchez-Vega F,Adusumilli PS,Schultz N,Jones DR
PA1-984B was used in Western Blotting to study the mechanism by which adenosine deaminase acting on double-stranded RNA facilitates the progression of lung adenocarcinoma.
Tue Sep 19 00:00:00 EDT 2017
GRWD1 negatively regulates p53 via the RPL11-MDM2 pathway and promotes tumorigenesis.
EMBO reports
Kayama K,Watanabe S,Takafuji T,Tsuji T,Hironaka K,Matsumoto M,Nakayama KI,Enari M,Kohno T,Shiraishi K,Kiyono T,Yoshida K,Sugimoto N,Fujita M
PA1984B was used in western blot to elucidate the mechanism by which GRWD1 regulates p53 levels
Sun Jan 01 00:00:00 EST 2017
Downregulation of the proapoptotic protein MOAP-1 by the UBR5 ubiquitin ligase and its role in ovarian cancer resistance to cisplatin.
Oncogene
Matsuura K,Huang NJ,Cocce K,Zhang L,Kornbluth S
PA1984B was used in immunoprecipitation to identify homologous to the E6-AP carboxyl terminus as a ubiquitin ligase for modulator of apoptosis protein 1
Thu Mar 23 00:00:00 EDT 2017
Secretory pathway Ca2+ -ATPases promote in vitro microcalcifications in breast cancer cells.
Molecular carcinogenesis
Dang D,Prasad H,Rao R
PA1-984B was used in Western Blotting to examine the molecular pathways that lead to microcalcifications in the breast.
Wed Nov 01 00:00:00 EDT 2017
Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.
The EMBO journal
Sellier C,Campanari ML,Julie Corbier C,Gaucherot A,Kolb-Cheynel I,Oulad-Abdelghani M,Ruffenach F,Page A,Ciura S,Kabashi E,Charlet-Berguerand N
PA1-984B was used in Western Blotting to indicate that partial loss of function of C9ORF72 synergizes with Ataxin-2 toxicity, suggesting a double-hit pathological mechanism in ALS-FTD.
Wed Jun 15 00:00:00 EDT 2016
IRBIT controls apoptosis by interacting with the Bcl-2 homolog, Bcl2l10, and by promoting ER-mitochondria contact.
eLife
Bonneau B,Ando H,Kawaai K,Hirose M,Takahashi-Iwanaga H,Mikoshiba K
PA1984B was used in western blot to determine that the antiapoptotic protein Bcl2l10 and IRBIT interact and exert an additive inhibition of inositol 1,4,5-trisphosphate receptor in the physiological state
Tue Dec 20 00:00:00 EST 2016
Disrupted-in-schizophrenia 1 (DISC1) and Syntaphilin collaborate to modulate axonal mitochondrial anchoring.
Molecular brain
Park C,Lee SA,Hong JH,Suh Y,Park SJ,Suh BK,Woo Y,Choi J,Huh JW,Kim YM,Park SK
PA1-984B was used in Western blotting following immunoprecipitation to study the regulation of neuronal activation-induced mitochondrial anchoring by Disrupted-in-schizophrenia 1.
Sat Jul 02 00:00:00 EDT 2016
Disrupted-in-schizophrenia 1 (DISC1) and Syntaphilin collaborate to modulate axonal mitochondrial anchoring.
Molecular brain
Park C,Lee SA,Hong JH,Suh Y,Park SJ,Suh BK,Woo Y,Choi J,Huh JW,Kim YM,Park SK
PA1-984B was used in Western blotting following immunoprecipitation to study the regulation of neuronal activation-induced mitochondrial anchoring by Disrupted-in-schizophrenia 1.
Sat Jul 02 00:00:00 EDT 2016
Stability of HIB-Cul3 E3 ligase adaptor HIB Is Regulated by Self-degradation and Availability of Its Substrates.
Scientific reports
Zhou Z,Xu C,Chen P,Liu C,Pang S,Yao X,Zhang Q
PA1-984B was used in immunohistochemistry to investigate the regulation of HIB
Wed Aug 12 00:00:00 EDT 2015
Palmitoylation of TNF alpha is involved in the regulation of TNF receptor 1 signalling.
Biochimica et biophysica acta
Poggi M,Kara I,Brunel JM,Landrier JF,Govers R,Bonardo B,Fluhrer R,Haass C,Alessi MC,Peiretti F
PA1-984B was used in immunocytochemistry to study the regulation of TNFR1 signaling by palmitoylation of TNFalpha
Fri Mar 01 00:00:00 EST 2013
Unregulated smooth-muscle myosin in human intestinal neoplasia.
Proceedings of the National Academy of Sciences of the United States of America
Alhopuro P,Phichith D,Tuupanen S,Sammalkorpi H,Nybondas M,Saharinen J,Robinson JP,Yang Z,Chen LQ,Orntoft T,Mecklin JP,Järvinen H,Eng C,Moeslein G,Shibata D,Houlston RS,Lucassen A,Tomlinson IP,Launonen V,Ristimäki A,Arango D,Karhu A,Sweeney HL,Aaltonen LA
PA1-984B was used in EMSA assay to investigate the changes of myh11 in human intestinal tumor
Tue Apr 08 00:00:00 EDT 2008
Transcriptional control of BubR1 by p53 and suppression of centrosome amplification by BubR1.
Molecular and cellular biology
Oikawa T,Okuda M,Ma Z,Goorha R,Tsujimoto H,Inokuma H,Fukasawa K
PA1-984B was used in western blot to study the regulatory control of BubR1 and the effect of BubR1 on centrosome amplification by BubR1.
Sun May 01 00:00:00 EDT 2005
Cysteine-scanning mutagenesis of serotonin transporter intracellular loop 2 suggests an alpha-helical conformation.
The Journal of biological chemistry
Zhang YW,Rudnick G
PA1-984B was used in immunocytochemistry to demonstrate a possible mechanism for the regulation of SERT activity.
Fri Sep 02 00:00:00 EDT 2005
Differences in endosomal targeting of human (beta)1- and (beta)2-adrenergic receptors following clathrin-mediated endocytosis.
Journal of cell science
Liang W,Curran PK,Hoang Q,Moreland RT,Fishman PH
PA1-984B was used in immunocytochemistry to study the internalization of adrenergic receptors.
Sun Feb 15 00:00:00 EST 2004
Pharmacological rescue of trafficking defective HERG channels formed by coassembly of wild-type and long QT mutant N470D subunits.
American journal of physiology. Heart and circulatory physiology
Gong Q,Anderson CL,January CT,Zhou Z
PA1-984B was used in immunocytochemistry to investigate defective HERG channels formed by N470D mutant and WT subunits and the rescue by the blocking drug E-4031
Sun Aug 01 00:00:00 EDT 2004
Critical role for protein phosphatase 2A heterotrimers in mammalian cell survival.
The Journal of biological chemistry
Strack S,Cribbs JT,Gomez L
PA1-984B was used in western blot to investigate the effect of protein phosphatase 2A on mammalian cell survial.
Fri Nov 12 00:00:00 EST 2004
Multiple type A/B heterogeneous nuclear ribonucleoproteins (hnRNPs) can replace hnRNP A1 in mouse hepatitis virus RNA synthesis.
Journal of virology
Shi ST,Yu GY,Lai MM
PA1-984B was used in western blot to investigate the interaction of CB3 intracellular proteins with mouse hepatitis virus RNA
Wed Oct 01 00:00:00 EDT 2003
