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DYKDDDDK Tag Antibody (701629RP488) in ICC/IF
Immunofluorescent analysis of DYKDDDDK Tag was performed using 70-80% confluent HA-H3-DYKDDDDK transfected HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were stained with DYKDDDDK Tag Recombinant Rabbit Monoclonal Antibody (8H8L17), Alexa Fluor™ Plus 488 (Prod... View More
产品信息
701629RP488
产品规格
种属反应
Tag
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
8H8L17
抗原
Synthetic peptide (DYKDDDDK)
偶联物
激发/发射光谱
493/518 nm
查看光谱
形式
Liquid
浓度
1.0 mg/mL
纯化类型
Protein A
保存液
proprietary buffer, pH 6.5
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
RRID
AB_2942243
产品详细信息
DYKDDDDK Tag recombinant rabbit monoclonal antibody, specifically recognizes DYKDDDDK Tag at the C-terminus but not N-terminus of proteins.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include better specificity and sensitivity, lot-to-lot consistency, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus antibodies. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor antibodies, providing you with better signal-to-noise for your critical experiments. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning images.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.
Applications Tested: This DYKDDDDK Tag antibody has been tested by immunoblotting, immunocytochemistry and flow cytometric analysis of cells transfected with a vector encoding DYKDDDDK Tag. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
