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Invitrogen
DYKDDDDK Tag Monoclonal Antibody (FG4R), HRP
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
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DYKDDDDK Tag Antibody (MA1-91878-HRP) in WB
Western Blot was performed using HRP-conjµgated Anti-DYKDDDDK Mouse Monoclonal Antibody (Product # MA1-91878-HRP) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), HA-H3-DYKDDDDK, 40 µg (Lane 3), HA-H3-DYKDDDDK, 20 µg (Lane 4), HA-H3-DYKDDDDK, 10 µg (Lane 5), HA-H3-DYKDDDDK, 5 µg (Lane 6), DYKDDDDK-p65-HA, 40 µg (Lane 7), Myc-p65-V5, 40 µg (Lane 8) and 25 ng of Positope (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris ... View More
产品信息
MA1-91878-HRP
产品规格
种属反应
Tag
已发表种属
Tag
宿主/亚型
Mouse
/ IgG2b
分类
Monoclonal
类型
Antibody
克隆号
FG4R
抗原
Synthetic peptide (DYKDDDDK) coupled to KLH.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.07% Kathon
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2537626
产品详细信息
similar epitope as the FLAG tag from Sigma
MA1-91878 detects Flag tag from various samples.
MA1-91878 has been successfully used in Western blot applications.
The MA1-91878 immunogen is synthetic peptide (DYKDDDDK) coupled to KLH.
靶标信息
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
