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DYKDDDDK Tag Antibody (MA1-142-A555) in ICC/IF
Immunofluorescent analysis of HeLa cells transfected with a construct containing a FLAG® Epitope Tag. Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 inPBS for 15 minutes at room temperature and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with AlexaFluor 555 conjugated FLAG-tag monoclonal antibody (Product # MA1-142-A55... View More
产品信息
MA1-142-A555
产品规格
种属反应
Tag
已发表种属
Tag
宿主/亚型
Rat
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
L5
抗原
DYKDDDDK-tagged mouse Langerin
偶联物
激发/发射光谱
553/568 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2610654
产品详细信息
similar epitope as the Flag tag from Sigma
靶标信息
DYKDDDDK tag, also known as FLAG tag, is a short peptide sequence that is commonly used as a protein tag to facilitate protein purification, detection, and localization. The tag consists of eight amino acids: Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys. It is often fused to the N- or C-terminus of a protein of interest, allowing for easy identification and isolation of the protein using anti-FLAG antibodies. The DYKDDDDK tag is widely used in molecular biology and biochemistry research, and has been employed in various applications, such as protein-protein interaction studies, protein expression analysis, and protein localization studies. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: DDDDK; DDDDK epitope tag; ECS epitope tag; ECS tag; FLAG; FLAG Tag
