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Invitrogen
Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 680
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Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A10038) in ICC/IF
Immunofluorescence analysis of Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 680 (Product # A10038) was performed using SH-SY5Y (positive model) and T-47D (negative model) cells stained with Nestin Monoclonal Antibody (10C2), eBioscience™ (Product # 14-9843-80). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 min... View More
产品信息
A10038
应用
建议稀释比
免疫印迹 (WB)
1:5,000-1:50,000
免疫细胞化学 (ICC/IF)
1:200-1:2,000
产品规格
种属反应
Mouse
宿主/亚型
Donkey
/ IgG
分类
Polyclonal
类型
Secondary Antibody
抗原
Gamma Immunoglobin
偶联物
激发/发射光谱
681/704 nm
查看光谱
形式
Liquid
浓度
2 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.5
内含物
5mM sodium azide
保存条件
4° C, store in dark
运输条件
Ambient
RRID
AB_11180593
靶标
IgG
交叉吸附
Against bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum
抗体形式
Whole Antibody
产品详细信息
This secondary antibody is designed for fluorescent Western blot detection on various near-infrared fluorescence instruments. This antibody can be used for multi-color and multiplexing detection when using other antibodies conjugated to compatible Alexa Fluor™ dyes and wavelengths. Other applications of this antibody include immunofluorescent and fluorescent imaging applications when using instrumentation with appropriate excitation and detection capabilities. This antibody shows minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat, and sheep serum proteins.
Product will be shipped at Room Temperature.
靶标信息
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
