Invitrogen

E2F3 Monoclonal Antibody (PG30)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

查看其他14个E2F3抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

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E2F3 Antibody (MA1-25333) in WB

Western blot was performed using Anti-E2F3 Mouse Monoclonal Antibody (Product # MA1-25333) and a 52 kDa bands corresponding to E2F3 was observed across cell line tested and increased upon treatment with Doxorubicin and Etoposide. Whole cell extracts (30 µg lysate) of U2OS (Lane 1), U2OS treated with Doxorubicin (300ng/ml for 12 Hours) (Lane 2), U2OS treated with Etoposide (6µg/ml for 12 Hours) (Lane 3) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocell... View More

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E2F3 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Anti-E2F3 Monoclonal Antibody (Product # MA1-25333), shows induced expression in U2OS cells upon Doxorubicin and Etoposide treatment. {TM}

产品信息

MA1-25333

应用

建议稀释比

已发表文章

免疫印迹 (WB)

4-8 µg/mL

免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

PG30

抗原

Recombinant human protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.3 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 1% BSA

内含物

15mM sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2293325

产品详细信息

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.

仅用于科研。不用于诊断过程。未经明确授权不得转售。