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Application
FIGURE: 1 / 15
E2F4 Antibody (PA5-99166) in ICC/IF
Immunocytochemistry analysis of E2F4 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with E2F4 polyclonal antibody (Product # PA5-99166) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-99166
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human E2F4(Accession Q16254), corresponding to amino acid residues V30-Q80.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2818099
Product Specific Information
Antibody detects endogenous levels of total E2F4.
Target Information
E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F-4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides Differential phosphorylation contributes to the microheterogeniety of E2F-4 (total aa 411-416, varies due to a trinucleotide repeat).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E2F transcription factor 4, p107/p130-binding; E2F-4; p107/p130-binding protein; Transcription factor E2F4
Gene Aliases: 2010111M04Rik; AI427446; E2F-4; E2F4
UniProt ID: (Human) Q16254, (Mouse) Q8R0K9
Entrez Gene ID: (Human) 1874, (Rat) 100360427, (Mouse) 104394
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
protein domain specific binding
promoter-specific chromatin binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
RNA metabolism protein
nucleic acid metabolism protein
general transcription factor
regulation of transcription involved in G1/S transition of mitotic cell cycle
epithelial cell development
transcription from RNA polymerase II promoter
cell volume homeostasis
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
blood circulation
organ morphogenesis
regulation of cell proliferation
motile cilium assembly
positive regulation of transcription from RNA polymerase II promoter
centriole assembly
multi-ciliated epithelial cell differentiation
transcription, DNA-templated
regulation of cell size
cilium assembly
mitotic cell cycle
regulation of transcription, DNA-templated
cell cycle
cell projection organization
regulation of cell cycle
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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