Invitrogen

E2F8 Polyclonal Antibody

E2F8 Antibody in Immunocytochemistry (ICC/IF)

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E2F8 Antibody (PA5-100878) in ICC/IF

Immunocytochemistry analysis of E2F8 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with E2F8 polyclonal antibody (Product # PA5-100878) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).

E2F8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-100878

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human E2F8(Accession A0AVK6), corresponding to amino acid residues Q807-R857.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Wet ice

RRID

AB_2850375

产品详细信息

Antibody detects endogenous levels of total E2F8.

靶标信息

E2F transcription factors, such as E2F8, are essential for orchestrating expression of genes required for cell cycle progression and proliferation (Christensen et al., 2005).

仅用于科研。不用于诊断过程。未经明确授权不得转售。