Invitrogen

EDC4 Polyclonal Antibody

EDC4 Antibody in Immunocytochemistry (ICC/IF)

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EDC4 Antibody (PA5-59461) in ICC/IF

Immunofluorescent analysis of DCP1A (green) in Hela cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 2% BSA in PBS for 60 minutes at room temperature. Cells were stained with a EDC4 polyclonal antibody (Product # MA5-15681) at a dilution of 2 µg/mL in staining buffer for 3 hour at room temperature, and then incubated with a Goat anti-rabbit IgG Secondary Antibody, Alexa Fluor 488 conjugate (Product # A32731) at a dilution of 1:1000 for 1 hour ... View More

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产品信息

PA5-59461

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

免疫组化 (IHC)

1:20-1:50

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human EDC4. Recombinant protein control fragment (Product #RP-98599).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.05 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2640885

产品详细信息

Immunogen sequence: VERALETRHE QEQRRLERAL AEGQQRGGQL QEQLTQQLSQ ALSSAVAGRL ERSIRDEIKK TVPPCVSRSL EPMAGQLSNS VATKLTAVEG SMKENISKLL KSKNLTDA

Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 99%.

靶标信息

In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro).

仅用于科研。不用于诊断过程。未经明确授权不得转售。