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EDD1 Antibody (A300-573A-T) in WB
Detection of human and mouse EDD1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EDD1 antibody A300-573A-M (lot A300-573A-M-5) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.
产品信息
A300-573A-T
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Between 600 and 650
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
TBS, pH 7.0 to 8.0
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Wet ice
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Rat
靶标信息
EDD (for E3 identified by Differential Display) is a progestin-regulated gene that was isolated from T-47D human breast cancer cells. Based on sequence homology, EDD appears to be a human homolog of the Drosophila hyperplastic discs (hyd) gene, a tumor suppressor gene that is required for control of imaginal disc growth. EDD contains a HECT domain in the carboxy terminus. HECT domain-containing proteins function as ubiquitin-protein ligases, or E3 enzymes. EDD has been shown to bind to ubiquitin, and like other HECT family proteins, may function as an E3 ubiquitin-protein ligase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CH D1S3362; E3 identified by differential display; E3 ubiquitin-protein ligase UBR5; E3 ubiquitin-protein ligase, HECT domain-containing 1; ECGF1; EDG-1; EDG1; extraembryonic development; hHYD; Hyperplastic discs protein homolog; Progestin-induced protein; S1P1; S1PR1
基因别名: DD5; EDD; EDD1; HYD; KIAA0896; UBR5
UniProt ID: (Human) O95071, (Mouse) Q80TP3
Entrez Gene ID: (Human) 51366, (Mouse) 70790
RNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
ubiquitin-ubiquitin ligase activity
ubiquitin binding
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein modifying enzyme
protein polyubiquitination
DNA repair
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
cell proliferation
positive regulation of protein import into nucleus, translocation
positive regulation of catenin import into nucleus
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
progesterone receptor signaling pathway
positive regulation of canonical Wnt signaling pathway
negative regulation of histone H2A K63-linked ubiquitination
negative regulation of double-strand break repair
