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EDF1 Antibody (PA5-142102) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of EDF1 in HeLa cells using EDF1 Polyclonal Antibody (Product # PA5-142102). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
产品信息
PA5-142102
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Peptide with sequence AESDWDTVTVLRK-C.
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Ammonium sulfate precipitation
保存液
TBS, pH 7.3, with 0.5% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2932763
产品详细信息
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.
靶标信息
This gene is known to suppress G-protein and mitogen-activated signal transduction in mammalian cells. The encoded protein shares significant similarity with Arabidopsis FUS6, which is a regulator of light-mediated signal transduction in plant cells. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EDF-1; Endothelial differentiation-related factor 1; hypothetical protein 1-9; MBF1; MGC9058; multiprotein bridging factor 1; Multiprotein-bridging factor 1
基因别名: 0610008L11Rik; AA409425; CFAP280; EDF-1; EDF1; MBF1
UniProt ID: (Human) O60869, (Mouse) Q9JMG1
Entrez Gene ID: (Human) 8721, (Mouse) 59022
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
cell differentiation
positive regulation of DNA binding
regulation of nucleic acid-templated transcription
regulation of lipid metabolic process
endothelial cell differentiation
positive regulation of transcription, DNA-templated
