Invitrogen

EGFR L858R Recombinant Rabbit Monoclonal Antibody (RM380), Biotin

查看其他3个EGFR (mutant L858R)抗体
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产品信息

MA5-44558

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000-1:2,000
-

免疫组化（石蜡） (IHC (P))

1:100-1:200
-

免疫细胞化学 (ICC/IF)

1:100
-
产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

RM380

抗原

A peptide corresponding to human EGFR (L858R Mutant Specific). The immunogen range is a synthetic peptide within amino acids 1-100 of histone H3.3 with G34R mutant.
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偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

100 µg/mL

纯化类型

Protein A

保存液

PBS with 1% BSA, 50% glycerol

内含物

0.09% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2926688

靶标信息

EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Phosphorylation of EGFR at certain residues is also mediated by Src-non-receptor kinase. EGFR activation signals multiple downstream signaling cascades such as the Ras - ERK, PI3-K - Akt, Jak - STAT and PKC pathways that help in growth and proliferation of cells. Phosphorylation of EGFR at Y1086 specifically allows binding of the adaptor protein GRB2, leading to activation of the MAPK pathway. Upon receptor activation and signaling, EGFR is endocytosed and targeted for degradation or recycling. Mutations in the EGFR gene are associated with lung cancer and multiple alternatively spliced transcript variants encode different protein isoforms of EGFR have been found. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. Moreover, EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. This product is specific for EGFR (mutant L858R).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

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基因别名: EGFR; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61

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UniProt ID: (Human) P00533

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Entrez Gene ID: (Human) 1956

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功能
chromatin binding double-stranded DNA binding MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding enzyme binding protein phosphatase binding nitric-oxide synthase regulator activity ubiquitin protein ligase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity actin filament binding cadherin binding involved in cell-cell adhesion transmembrane signal receptor
参与通路
MAPK cascade activation of MAPKK activity cell morphogenesis ossification embryonic placenta development positive regulation of protein phosphorylation hair follicle development response to stress signal transduction cell surface receptor signaling pathway epidermal growth factor receptor signaling pathway activation of phospholipase C activity salivary gland morphogenesis learning or memory cell proliferation positive regulation of cell proliferation regulation of phosphatidylinositol 3-kinase signaling single organismal cell-cell adhesion peptidyl-tyrosine phosphorylation cerebral cortex cell migration positive regulation of cell growth positive regulation of cell migration positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle positive regulation of catenin import into nucleus ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway negative regulation of protein catabolic process positive regulation of phosphorylation activation of phospholipase A2 activity by calcium-mediated signaling negative regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of GTPase activity positive regulation of nitric oxide biosynthetic process positive regulation of DNA repair positive regulation of DNA replication positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation digestive tract morphogenesis positive regulation of epithelial cell proliferation regulation of peptidyl-tyrosine phosphorylation regulation of nitric-oxide synthase activity protein insertion into membrane positive regulation of protein kinase B signaling morphogenesis of an epithelial fold eyelid development in camera-type eye response to UV-A positive regulation of ERK1 and ERK2 cascade cellular response to amino acid stimulus cellular response to epidermal growth factor stimulus cellular response to estradiol stimulus positive regulation of production of miRNAs involved in gene silencing by miRNA regulation of cell motility
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