Invitrogen

EIF2S1 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3个已发表图片

3 篇参考文献

查看其他30个EIF2S1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

EIF2S1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 9

EIF2S1 Antibody (PA5-27366) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of eIF2a was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF2a Polyclonal Antibody (Product # PA5-27366) diluted at 1:5000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

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产品信息

PA5-27366

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

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免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 315 of Human EIF2 alpha

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.63 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2544842

产品详细信息

Recommended positive controls: A549, H1299, HCT-116, rat neuron, HeLa, N2a.

Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (92%), Chicken (97%), Rhesus Monkey (100%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Protective Effect of Anthocyanin-Enriched Polyphenols from Hibiscus syriacus L. (Malvaceae) against Ultraviolet B-Induced Damage.

Antioxidants (Basel, Switzerland)

Karunarathne WAHM,Molagoda IMN,Lee KT,Choi YH,Yu SM,Kang CH,Kim GY

PA5-27366 was used in Western Blotting to investigate the protective effect of AHs against UVB-induced damage.

Fri Apr 09 00:00:00 EDT 2021

Protective Effect of Anthocyanin-Enriched Polyphenols from Hibiscus syriacus L. (Malvaceae) against Ultraviolet B-Induced Damage.

Antioxidants (Basel, Switzerland)

Karunarathne WAHM,Molagoda IMN,Lee KT,Choi YH,Yu SM,Kang CH,Kim GY

PA5-27366 was used in Western Blotting to investigate the protective effect of AHs against UVB-induced damage.

Fri Apr 09 00:00:00 EDT 2021

Fisetin Protects HaCaT Human Keratinocytes from Fine Particulate Matter (PM_2.5)-Induced Oxidative Stress and Apoptosis by Inhibiting the Endoplasmic Reticulum Stress Response.

Antioxidants (Basel, Switzerland)

Molagoda IMN,Kavinda MHD,Choi YH,Lee H,Kang CH,Lee MH,Lee CM,Kim GY

PA5-27366 was used in Western Blotting to suggest that fisetin inhibits PM2.5-induced apoptosis by inhibiting the ER stress response and production of ROS.

Sat Sep 18 00:00:00 EDT 2021

Fisetin Protects HaCaT Human Keratinocytes from Fine Particulate Matter (PM_2.5)-Induced Oxidative Stress and Apoptosis by Inhibiting the Endoplasmic Reticulum Stress Response.

Antioxidants (Basel, Switzerland)

Molagoda IMN,Kavinda MHD,Choi YH,Lee H,Kang CH,Lee MH,Lee CM,Kim GY

PA5-27366 was used in Western Blotting to suggest that fisetin inhibits PM2.5-induced apoptosis by inhibiting the ER stress response and production of ROS.

Sat Sep 18 00:00:00 EDT 2021

ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer.

Journal of biomedical science

Cheung CHY,Hsu CL,Lin TY,Chen WT,Wang YC,Huang HC,Juan HF

Published figure using EIF2S1 polyclonal antibody (Product # PA5-27366) in Western Blot

Tue Jun 23 00:00:00 EDT 2020

ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer.

Journal of biomedical science

Cheung CHY,Hsu CL,Lin TY,Chen WT,Wang YC,Huang HC,Juan HF

Published figure using EIF2S1 polyclonal antibody (Product # PA5-27366) in Western Blot

Tue Jun 23 00:00:00 EDT 2020

生物信息学

蛋白别名: eIF-2-alpha; EIF-2A; EIF-2alpha; eIF2 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 2A

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基因别名: 0910001O23Rik; 2410026C18Rik; 35kDa; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; eIF2alpha; EIF2S1

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UniProt ID: (Human) P05198, (Rat) P68101, (Mouse) Q6ZWX6

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Entrez Gene ID: (Human) 1965, (Rat) 54318, (Mouse) 13665

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功能

translation initiation factor activity protein binding ribosome binding poly(A) RNA binding nucleic acid binding RNA binding translation initiation factor

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