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Invitrogen
EIF2S1 Recombinant Rabbit Monoclonal Antibody (PSH04-29)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 10
EIF2S1 Antibody (MA5-52465) in ICC/IF
Immunofluorescence analysis of EIF2S1 using HepG2 cells. The cells were fixed 4% paraformaldehyde for 20 minutes, permeabilized with 0.1% Triton™ X-100 in PBS for 5 minutes, and blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. The cells were co-labeled with EIF2S1 Recombinant Rabbit Monoclonal Antibody (PSH04-29) (Product # MA5-52465) (green) and beta tubulin (red) at 1:100 dilution in in 1% BSA in PBST overnight at 4 degrees Celsius and then with iFluor™ 488 Goat Anti-Rabbit IgG H&L and iFluor™ 594 Goat Anti-... View More
产品信息
MA5-52465
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH04-29
抗原
Recombinant protein within human eif2s1 aa 1-315/315.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1 BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
产品详细信息
Positive Control: MCF7 cell lysate, HepG2 cell lysate, HeLa cell lysate, COS-1 cell lysate, A549 cell lysate, RAW264.7 cell lysate, C6 cell lysate, mouse kidney tissue lysate, mouse spleen tissue lysate, rat kidney tissue lysate, rat spleen tissue lysate, HepG2, human breast tissue, mouse breast tissue, rat breast tissue.
Sequence Similarities: 99% Mouse/Rat.
Subcellular Location: Cytoplasm, Stress granule.
Predicted band size: 36 kDa.
靶标信息
EIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of initiator methionyl-tRNA to the 40S ribosome in an AUG-dependent manner to form the 43S pre-initiation complex. The polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: eIF-2-alpha; EIF-2A; EIF-2alpha; eIF2 alpha; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa; eukaryotic translation initiation factor 2A
基因别名: 0910001O23Rik; 2410026C18Rik; 35kDa; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; eIF2alpha; EIF2S1
UniProt ID: (Human) P05198, (Rat) P68101, (Mouse) Q6ZWX6
Entrez Gene ID: (Human) 1965, (Rat) 54318, (Mouse) 13665
positive regulation of defense response to virus by host
translational initiation
aging
negative regulation of translational initiation in response to stress
cellular response to amino acid starvation
cellular response to heat
cellular response to UV
response to endoplasmic reticulum stress
PERK-mediated unfolded protein response
protein autophosphorylation
transmembrane transport
mitophagy in response to mitochondrial depolarization
xenophagy
positive regulation of neuron death
response to manganese-induced endoplasmic reticulum stress
positive regulation of type B pancreatic cell apoptotic process
translation
regulation of translational initiation in response to stress
regulation of translation
