Invitrogen

ENDOG Polyclonal Antibody

ENDOG Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ENDOG Antibody (PA5-20004) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using ENDOG Polyclonal Antibody (Product # PA5-20004) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as seconda... View More

ENDOG Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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产品信息

PA5-20004

应用

建议稀释比

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免疫印迹 (WB)

2 µg/mL

免疫组化（石蜡） (IHC (P))

2.5-15 µg/mL

免疫组织化学（多聚甲醛固定） (IHC (PFA))

2.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11157676

产品详细信息

In Western blot applications, this antibody detects a band at ~35kDa. A suggested positive control is HepG2 cell lysate.

PA5-20004 can be used with blocking peptide PEP-0126.

靶标信息

The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.

仅用于科研。不用于诊断过程。未经明确授权不得转售。