Invitrogen

ENDOG Recombinant Rabbit Monoclonal Antibody (JU31-30)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他12个ENDOG抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (7)
高级验证 (1)

ENDOG Antibody in Immunocytochemistry (ICC/IF)

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ENDOG Antibody (MA5-34629) in ICC/IF

Immunofluorescence analysis of ENDOG was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ENDOG Recombinant Rabbit Monoclonal Antibody (JU31-30) (Product # MA5-34629) at 1:100 dilution in 0.1% BSA, incubated at 4 degrees celsius overnight, and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fl... View More

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ENDOG Antibody — Antibody specificity was demonstrated by siRNA-mediated knockdown of the target protein. Hep G2 cells were transfected with ENDOG siRNA and a decrease in signal intensity was observed in Western Blot application using Anti-ENDOG Recombinant Rabbit Monoclonal Antibody (JU31-30) (Product # MA5-34629). {KD}

产品信息

MA5-34629

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JU31-30

抗原

Synthetic peptide within Human Endo G aa 200-297

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles, store in dark

运输条件

Wet ice

RRID

AB_2848537

产品详细信息

Positive Control: MCF-7, PC-3M, SH-SY5Y, mouse heart tissue lysate.

靶标信息

The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.

仅用于科研。不用于诊断过程。未经明确授权不得转售。