Invitrogen

EOMES Monoclonal Antibody (21Mags8), eBioscience™

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2个已发表图片

5 篇参考文献

查看其他34个EOMES抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 4

EOMES Antibody (14-4876-82) in WB

Mouse spleen cell lysate were resolved by SDS-PAGE and electroblotted onto a PVDF membrane. The membrane was probed with 2 µg/mL of Anti-Human/Mouse EOMES Purified and revealed using an Anti-Rat IgG HRP antibody.

Product Image Gallery

EOMES Antibody — Figure 5 T-bet-deficient T cells are hyper-responsive to IL-23. C57BL/6 Rag1 -/- mice were injected i.p. with either WT or Tbx21 -/- CD4 + CD25 - CD45RB hi T cells or a 1:1 mixtures of WT (CD45.1 + ) and Tbx21 -/- (CD45.2 + ) CD4 + CD25 - CD45RB hi T cells. Mice were killed when recipients of Tbx21 -/- T cells developed clinical signs of disease (~5 weeks) and assessed for intestinal inflammation. ( a ) Colitis scores. ( b ) Frequencies of IL-17A + and/or IFN-gamma + cells in the colon of mice receiving mix of WT and Tbx21 -/- CD4 + T cells. ( c ) CD4 + T cells were purified by flow cytometry from the inflamed colon of mice receiving a mix of WT and Tbx21 -/- CD4 + T cells and mRNA levels of indicated genes were analysed by quantitative reverse transcription PCR (qRT-PCR). ( d ) ChIP-qRT-PCR for the occupancy of H3K4me3 or RNA Pol II around the TSS of indicated genes in CD4 + T cells purified as described in c . ( e ) Immunoblot for pY705-STAT3, total STAT3, EOMES, Runx3 and Actin on whole cells lysates of CD4 + T cells purified as in c , left unstimulated or stimulated with IL-23 for 12 h. ( f ) Concentrations of indicated cytokines secreted into culture medium by CD4 + T cells purified and stimulated as described in e . Data in a - c and f represent results from one of two independent experiments ( n =5 for WT, n =6 for Tbx21 -/- , n =6 for WT+ Tbx21 -/- ). Bars are the mean and each symbol represents an individual mouse. Bars are the mean and error bars represent s.e.m. Da {TM}

产品信息

14-4876-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

查看2篇已发表文章 2篇已发表文章

流式细胞分析 (Flow)

Assay-Dependent

染色质免疫沉淀实验 (ChIP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

21Mags8

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1210517

产品详细信息

Description: This 21Mags8 antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Note: For detection of mouse Eomes, we recommend 12-4875 and 51-4875 and for detection of human Eomes, we recommend 51-4877 due to the brighter staining intensity of these products compared to clone 21Mags8.

Applications Reported: This 21Mags8 antibody has been reported for use in flow cytometric analysis, and immunoblotting (WB).

Applications Tested: This 21Mags8 antibody has been tested by western blot analysis of mouse splenocytes. As a starting concentration, this antibody can be used at 2 µg/mL.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

T-bet is a key modulator of IL-23-driven pathogenic CD4(+) T cell responses in the intestine.

Nature communications

Krausgruber T,Schiering C,Adelmann K,Harrison OJ,Chomka A,Pearson C,Ahern PP,Shale M,Oukka M,Powrie F

14-4876 was used in Western Blotting to identify T-bet as a key modulator of IL-23-driven colitogenic responses in the intestine.

Thu May 19 00:00:00 EDT 2016

TBX3 Directs Cell-Fate Decision toward Mesendoderm.

Stem cell reports

Weidgang CE,Russell R,Tata PR,Kühl SJ,Illing A,Müller M,Lin Q,Brunner C,Boeckers TM,Bauer K,Kartikasari AE,Guo Y,Radenz M,Bernemann C,Weiß M,Seufferlein T,Zenke M,Iacovino M,Kyba M,Schöler HR,Kühl M,Liebau S,Kleger A

14487682 was used in immunocytochemistry to report that TBX3 is an upstream regulator of the mesendoderm transcriptional program during gastrulation

Mon May 11 00:00:00 EDT 2015

Unique features of naive CD8+ T cell activation by IL-2.

Journal of immunology (Baltimore, Md. : 1950)

Cho JH,Kim HO,Kim KS,Yang DH,Surh CD,Sprent J

14-4876 was used in Western Blotting to demonstrate that IL-2 elicits a unique signaling pattern associated with strong lymphocyte-specific protein tyrosine kinase/JAK3-dependent activation of the PI3K/AKT pathway.

Sun Dec 01 00:00:00 EST 2013

The T-box transcription factor Eomesodermin acts upstream of Mesp1 to specify cardiac mesoderm during mouse gastrulation.

Nature cell biology

Costello I,Pimeisl IM,Dräger S,Bikoff EK,Robertson EJ,Arnold SJ

14-4876 was used in Immunofluorescence to show that Eomes governs transcriptional programmes that sequentially specify cardiac and definitive endoderm progenitors during gastrulation.

Sun Aug 07 00:00:00 EDT 2011

The T-box transcription factor Eomesodermin acts upstream of Mesp1 to specify cardiac mesoderm during mouse gastrulation.

Nature cell biology

Costello I,Pimeisl IM,Dräger S,Bikoff EK,Robertson EJ,Arnold SJ

14-4876 was used in Immunofluorescence to show that Eomes governs transcriptional programmes that sequentially specify cardiac and definitive endoderm progenitors during gastrulation.

Sun Aug 07 00:00:00 EDT 2011

生物信息学

蛋白别名: Eomesodermin homolog; T-box brain protein 2; T-brain-2

查看更多收起

基因别名: C77258; EOMES; TBR-2; TBR2

查看更多收起

UniProt ID: (Human) O95936, (Mouse) O54839

查看更多收起

Entrez Gene ID: (Human) 8320, (Mouse) 13813

查看更多收起

功能

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding Rel homology transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter endoderm formation mesoderm formation endodermal cell fate specification trophectodermal cell differentiation adaptive immune response CD8-positive, alpha-beta T cell differentiation involved in immune response transcription, DNA-templated brain development cardioblast differentiation stem cell population maintenance olfactory bulb development cerebral cortex regionalization cerebral cortex neuron differentiation interferon-gamma production skeletal muscle cell differentiation positive regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell differentiation involved in embryonic placenta development mesodermal to mesenchymal transition involved in gastrulation blastocyst development immune system process regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development gastrulation regulation of gene expression cell differentiation regulation of neurogenesis

EOMES Monoclonal Antibody (21Mags8), eBioscience™

EOMES Antibody (14-4876-82) in WB

产品信息

14-4876-82

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

EOMES Monoclonal Antibody (21Mags8), eBioscience™

EOMES Antibody (14-4876-82) in WB

产品信息

14-4876-82

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)