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ERO1LB Antibody (PA5-100065) in ICC/IF
Immunofluorescent analysis of ERO1LB in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ERO1LB polyclonal antibody (Product # PA5-100065) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
产品信息
PA5-100065
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human ERO1B(Accession Q86YB8), corresponding to amino acid residues W196-F246.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2815595
产品详细信息
Antibody detects endogenous levels of total ERO1LB.
靶标信息
ERO1LB is essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. ERO1LB acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. ERO1LB does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: endoplasmic oxidoreductase 1 beta; endoplasmic oxidoreductin-1-like protein B; Endoplasmic reticulum oxidoreductase beta; Endoplasmic reticulum oxidoreductin-1-like protein B; ERO1-L-beta; ERO1-like beta; ERO1-like protein beta; Oxidoreductin-1-L-beta
基因别名: 1300013B24Rik; 1700065B09Rik; AI447560; ero1-beta; ERO1B; Ero1beta; ERO1LB; RGD1563548
UniProt ID: (Human) Q86YB8, (Mouse) Q8R2E9
Entrez Gene ID: (Human) 56605, (Mouse) 67475, (Rat) 364755
protein disulfide isomerase activity
protein binding
protein disulfide oxidoreductase activity
oxidoreductase activity
oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor
unfolded protein binding
peptide-methionine (S)-S-oxide reductase activity
peptide-methionine (R)-S-oxide reductase activity
methionine-R-sulfoxide reductase activity
oxidoreductase
