Invitrogen

EZH1 Recombinant Rabbit Monoclonal Antibody (PS01-09)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他11个EZH1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (8)
高级验证 (2)

EZH1 Antibody in Immunocytochemistry (ICC/IF)

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EZH1 Antibody (MA5-46620) in ICC/IF

Immunofluorescence analysis of EZH1 was performed using 70% confluent log phase SK-MEL-5 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Anti-EZH1 Recombinant Rabbit Monoclonal Antibody (PS01-09) (Product # MA5-46620) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alex... View More

EZH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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EZH1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Reh cells were transfected with EZH1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-EZH1 Recombinant Rabbit Monoclonal Antibody (PS01-09) (Product # MA5-46620). {KD}

产品信息

MA5-46620

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:2,000

免疫细胞化学 (ICC/IF)

1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PS01-09

抗原

Synthetic peptide within human EZH1 aa 471-510.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2937692

产品详细信息

Positive Control: HCT116 cell lysate, K562 cell lysate, PANC-1 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, rat large intestine tissue, human colon tissue, mouse large intestine tissue.

Subcellular Location: Nucleus.

Tissue Specificity: Tissue enhanced (retina).

Sequence Similarities: 97% Mouse/Rat.

靶标信息

EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 ).

仅用于科研。不用于诊断过程。未经明确授权不得转售。