Invitrogen

Endothelin 1 Monoclonal Antibody (ENDO20-2101.70)

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Endothelin 1 Antibody in Neutralization (Neu)

Endothelin 1 Antibody in Neutralization (Neu)

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Endothelin 1 Antibody (MA1-10818) in Neu

BMC cancer 2020 - Fig. 3 Breast cancer conditioned medium induces hypertrophy in human cardiomyocytes through endothlin-1 signaling pathway. a Representative images of cardiomyocytes stained with WGA (green) and DAPI (blue), scale bar = 50 mum, b Quantification of cell surface area in cardiomyocytes treated with either regular serum free medium or breast cancer MCF7 CM with or without the antibody against ET-1 (n = 70 per gr... View More

Endothelin 1 Antibody in Neutralization (Neu)

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Endothelin 1 Antibody in Neutralization (Neu) — Fig. 3 Breast cancer conditioned medium induces hypertrophy in human cardiomyocytes through endothlin-1 signaling pathway. a Representative images of cardiomyocytes stained with WGA (green) and DAPI (blue), scale bar = 50 mum, b Quantification of cell surface area in cardiomyocytes treated with either regular serum free medium or breast cancer MCF7 CM with or without the antibody against ET-1 (n = 70 per group). c beta-MHC mRNA levels that were normalized to beta-ACTIN in cardiomyocytes treated with either regular serum free medium or breast cancer MCF7 CM with or without antibodies against ET-1 (n = 6 per group), d Representative images of cardiomyocytes stained with WGA (green) and DAPI (blue), scale bar = 50 mum, e Quantification of cell surface area levels in cardiomyocytes treated with either regular serum free medium and breast cancer MCF7 CM with or without BQ-1 (n = 70 per group), f Cardiomyocytes were stained with WGA (green) and DAPI (blue), scale bar = 50 mum, g Quantification of cell surface area in cardiomyocytes treated with regular serum free medium, synthetic ET-1, breast cancer ZR75-1 CM or breast cancer T47D CM. h beta-MHC mRNA levels that were normalized to beta-ACTIN in cardiomyocytes treated with regular serum free medium, synthetic ET-1, breast cancer ZR75-1 CM or breast cancer T47D CM (n = 6 per group). i Representative images of cardiomyocytes stained with WGA (green) and DAPI (blue), scale bar = 50 mum, j Quantification of cell surface area in cardiom

产品信息

MA1-10818

应用

建议稀释比

已发表文章

免疫组化 (IHC)

1-10 µg/mL

-

酶联免疫吸附实验 (ELISA)

5-20 µg/mL

-

中和实验 (Neu)

-

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放射免疫测定 (RIA)

1-10 µg/mL

-

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

ENDO20-2101.70

抗原

Human endothelin 1.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.29 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1074213

产品详细信息

MA1-10818 detects Endothelin from human samples.

MA1-10818 has been successfully used in ELISA, radioimmune assay, and immunohistochemistry applications.

The MA1-10818 immunogen is Human endothelin 1.

靶标信息

The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Breast cancer diagnosis is associated with relative left ventricular hypertrophy and elevated endothelin-1 signaling.

BMC cancer

Maayah ZH,Takahara S,Alam AS,Ferdaoussi M,Sutendra G,El-Kadi AOS,Mackey JR,Pituskin E,Paterson DI,Dyck JRB

MA1-10818 was used in Functional assay to suggest that breast cancer cells play a greater role in inducing structural cardiac remodeling than previously appreciated and that tumor-derived endothelin-1 may play a pivotal role in this process.

Wed Aug 12 00:00:00 EDT 2020

Breast cancer diagnosis is associated with relative left ventricular hypertrophy and elevated endothelin-1 signaling.

BMC cancer

Maayah ZH,Takahara S,Alam AS,Ferdaoussi M,Sutendra G,El-Kadi AOS,Mackey JR,Pituskin E,Paterson DI,Dyck JRB

MA1-10818 was used in Functional assay to suggest that breast cancer cells play a greater role in inducing structural cardiac remodeling than previously appreciated and that tumor-derived endothelin-1 may play a pivotal role in this process.

Wed Aug 12 00:00:00 EDT 2020

生物信息学

蛋白别名: Big endothelin 1; Big ET; Big ET 1; Big ET1; BigET; Endothelin-1; Et; PPET1; Preproendothelin-1

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基因别名: ARCND3; EDN1; ET1; HDLCQ7; PPET1; QME

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UniProt ID: (Human) P05305

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Entrez Gene ID: (Human) 1906

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cytokine activity hormone activity protein binding endothelin A receptor binding endothelin B receptor binding peptide hormone

参与通路

negative regulation of transcription from RNA polymerase II promoter prostaglandin biosynthetic process patterning of blood vessels in utero embryonic development histamine secretion regulation of systemic arterial blood pressure by endothelin regulation of pH cell surface receptor signaling pathway G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration protein kinase C-activating G-protein coupled receptor signaling pathway cell-cell signaling heart development respiratory gaseous exchange body fluid secretion positive regulation of cell proliferation dorsal/ventral pattern formation response to ozone multicellular organism aging positive regulation of heart rate positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy positive regulation of receptor biosynthetic process neural crest cell development phosphatidylinositol 3-kinase signaling response to activity artery smooth muscle contraction vein smooth muscle contraction glucose transport cell growth regulation of vasoconstriction sensory perception of pain calcium-mediated signaling peptide hormone secretion nitric oxide transport negative regulation of blood coagulation positive regulation of cell migration neutrophil chemotaxis negative regulation of cAMP biosynthetic process phospholipase D-activating G-protein coupled receptor signaling pathway negative regulation of cellular protein metabolic process positive regulation of prostaglandin secretion response to lipopolysaccharide response to testosterone negative regulation of smooth muscle cell apoptotic process response to prostaglandin F response to nicotine cellular response to drug positive regulation of urine volume positive regulation of renal sodium excretion response to muscle stretch epithelial fluid transport vasoconstriction protein kinase C deactivation middle ear morphogenesis positive regulation of odontogenesis superoxide anion generation rhythmic excitation response to amino acid positive regulation of MAP kinase activity positive regulation of JUN kinase activity response to leptin leukocyte activation positive regulation of nitric oxide biosynthetic process positive regulation of cell size positive regulation of mitotic nuclear division positive regulation of transcription from RNA polymerase II promoter positive regulation of smooth muscle contraction positive regulation of hormone secretion negative regulation of hormone secretion inositol phosphate-mediated signaling positive regulation of smooth muscle cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity cartilage development positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway negative regulation of nitric-oxide synthase biosynthetic process membrane depolarization regulation of sensory perception of pain maternal process involved in parturition positive regulation of sarcomere organization positive regulation of prostaglandin-endoperoxide synthase activity positive regulation of cell growth involved in cardiac muscle cell development positive regulation of chemokine-mediated signaling pathway cellular response to calcium ion cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to peptide hormone stimulus cellular response to glucocorticoid stimulus cellular response to mineralocorticoid stimulus cellular response to fatty acid cellular response to hypoxia response to dexamethasone cellular response to transforming growth factor beta stimulus response to salt