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Eotaxin Antibody (500-P67BT-25UG) in WB
Western Blot: To detect mEotaxin by Western Blot analysis Eotaxin Polyclonal Antibody, Biotin (Product # 500-P67BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P67BT-25UG
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Murine Eotaxin (CCL11)
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930699
产品详细信息
AA Sequence of recombinant protein: HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine Eotaxin (CCL11). Anti-Murine Eotaxin (CCL11)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect mEotaxin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine Eotaxin (500-P67) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant mEotaxin.
Western Blot: To detect mEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mEotaxin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P67BT-1MG will be provided as 2 x 500 µg
靶标信息
This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil specific chemokine assumed to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections.
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生物信息学
蛋白别名: C-C motif chemokine 11; Eosinophil chemotactic protein; Eotaxin; Eotaxin 1; Eotaxin1; MGC22554; small chemokine (C-C motif) ligand 11; small inducible cytokine A11; Small-inducible cytokine A11
基因别名: Ccl11; eotaxin; Scya11
UniProt ID: (Mouse) P48298
Entrez Gene ID: (Mouse) 20292
positive regulation of endothelial cell proliferation
mast cell chemotaxis
chemotaxis
inflammatory response
immune response
cytoskeleton organization
actin filament organization
regulation of cell shape
positive regulation of cell migration
positive regulation of actin filament polymerization
positive regulation of GTPase activity
positive regulation of angiogenesis
eosinophil chemotaxis
branching involved in mammary gland duct morphogenesis
mammary duct terminal end bud growth
ERK1 and ERK2 cascade
