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EphB4 Antibody (PA5-47013) in ICC/IF
Immunofluorescence analysis of EphB4 was performed using 70% confluent log phase C2C12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EphB4 Goat Polyclonal Antibody (Product # PA5-47013) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11078) at a dilution ... View More
产品信息
PA5-47013
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
宿主/亚型
Goat
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Mouse myeloma cell line NS0-derived recombinant mouse EphB4 Leu16-Ala539
偶联物
形式
Lyophilized
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5% trehalose
内含物
No Preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2608521
产品详细信息
In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) EphB6 and rmEphA3 is observed and less than 1% cross-reactivity with rmEphA2, rmEphA4, rmEphA6, rmEphA7, rmEphA8, rmEphB2, and rmEphB3 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
靶标信息
EPHB4 is a receptor tyrosine kinase that belongs to the ephrin receptor family. Members of the Eph family of kinases play important roles in diverse biological processes including nervous system development, angiogenesis and neural synapsis formation and maturation. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by EphB4 binds to ephrin-B2 and plays an essential role in vascular development.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Developmental kinase 2; ephrin receptor EphB4; Ephrin type-B receptor 4; Hepatoma transmembrane kinase; mDK-2; soluble EPHB4 variant 1; soluble EPHB4 variant 2; soluble EPHB4 variant 3; Tyrosine kinase MYK-1; tyrosine-protein kinase receptor HTK; Tyrosine-protein kinase TYRO11
基因别名: AI042935; EPHB4; HTK; MDK2; MYK1; TYRO11
UniProt ID: (Human) P54760, (Mouse) P54761
Entrez Gene ID: (Human) 2050, (Mouse) 13846
transmembrane receptor protein tyrosine kinase activity
ephrin receptor activity
protein binding
ATP binding
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
transmembrane signal receptor
angiogenesis
cell migration involved in sprouting angiogenesis
heart morphogenesis
cell adhesion
peptidyl-tyrosine phosphorylation
protein autophosphorylation
ephrin receptor signaling pathway
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
phosphorylation
