Invitrogen

Epiregulin Polyclonal Antibody

Epiregulin Antibody in Western Blot (WB)

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Epiregulin Antibody (PA5-47057) in WB

Western blot analysis of Epiregulin in P19 mouse embryonal carcinoma cell line. Samples were incubated in Epiregulin polyclonal antibody (Product # PA5-47057) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Proepiregulin at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions.

Epiregulin Antibody in Neutralization (Neu)

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产品信息

PA5-47057

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

中和实验 (Neu)

0.3-1.0 µg/mL

产品规格

种属反应

Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived recombinant mouse Epiregulin Val56-Leu101

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2607378

产品详细信息

In direct ELISAs, approximately 40% cross-reactivity with recombinant human Epiregulin is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

靶标信息

Epiregulin is an EGF-related growth factor that binds specifically to EGFR (ErbB1) and ErbB4, but not ErbB2 or ErbB3. It is expressed mainly in the placenta and peripheral blood leukocytes, as well as in certain carcinomas of the bladder, lung, kidney and colon. Epiregulin stimulates the proliferation of keratinocytes, hepatocytes, fibroblasts and vascular smooth muscle cells. It also inhibits the growth of several tumor-derived epithelial cell lines. Human Epiregulin is initially synthesized as a glycosylated 19.0 kDa transmembrane precursor protein, which is processed by proteolytic cleavage to produce a 6.0 kDa mature secreted sequence.

仅用于科研。不用于诊断过程。未经明确授权不得转售。