Invitrogen

FASN Monoclonal Antibody (GT237)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他32个FASN抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (9)
高级验证 (3)

FASN Antibody in Immunocytochemistry (ICC/IF)

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FASN Antibody (MA5-27744) in ICC/IF

Immunofluorescence analysis of FASN was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FASN Monoclonal Antibody (GT237) (Product # MA5-27744) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766... View More

FASN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FASN Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HepG2 cells were transfected with FASN siRNA and decrease in signal intensity was observed in Western Blot application using Anti-FASN Monoclonal Antibody (GT237) (Product # MA5-27744). {KD}

产品信息

MA5-27744

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:10,000

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

GT237

抗原

Recombinant protein encompassing a sequence within the N-terminus region of human Fatty Acid Synthase. The exact sequence is proprietary.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

no preservative

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2735179

产品详细信息

Positive Control: 293T, A431, HepG2, Neuro 2A, GL261, PC-12, Rat2

Predicted Reactivity: Pig (80%), Bovine (81%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

仅用于科研。不用于诊断过程。未经明确授权不得转售。