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Application
FIGURE: 1 / 1
FBXL10 Antibody (PA5-116649) in WB
Western blot analysis of FBXL10 in extracts of various cell lines. Samples were incubated with FBXL10 Polyclonal antibody (Product # PA5-116649) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-116649
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 600-700 of human KDM2B (XP_011537177.1)
Conjugate
Unconjugated
Form
Liquid
Concentration
1.17 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2901280
Product Specific Information
Positive Samples: HeLa, HepG2
Immunogen sequence: SGCSWIAVSA LCSSSCPLLR TLDVQWVEGL KDAQMRDLLS PPTDNRPGQM DNRSKLRNIV ELRLAGLDIT DASLRLIIRH MPLLSKLHLS YCNHVTDQSI N
Target Information
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs, which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [Histone-H3]-lysine-36 demethylase 1B; CXXC-type zinc finger protein 2; F-box and leucine-rich repeat protein 10; F-box protein FBL10; F-box/LRR-repeat protein 10; JEMMA (Jumonji domain, EMSY-interactor, methyltransferase motif) protein; JmjC domain-containing histone demethylation protein 1B; jumonji C domain-containing histone demethylase 1B; Jumonji domain-containing EMSY-interactor methyltransferase motif protein; lysine (K)-specific demethylase 2B; Lysine-specific demethylase 2B; Protein JEMMA; Protein-containing CXXC domain 2
Gene Aliases: CXXC2; FBL10; FBXL10; JHDM1B; KDM2B; NDY1; PCCX2
UniProt ID: (Human) Q8NHM5
Entrez Gene ID: (Human) 84678
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
spermatogenesis
midbrain-hindbrain boundary morphogenesis
fourth ventricle development
lateral ventricle development
third ventricle development
initiation of neural tube closure
positive regulation of cell growth
forebrain development
midbrain development
hindbrain development
histone H2A monoubiquitination
negative regulation of neuron apoptotic process
embryonic camera-type eye morphogenesis
oxidation-reduction process
histone H3-K36 demethylation
positive regulation of stem cell population maintenance
negative regulation of neural precursor cell proliferation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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