Invitrogen

FGF basic Monoclonal Antibody, PeproTech®

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FGF basic Antibody (500-M38C-500UG) in WB

Western Blot: To detect Human FGF-Basic by Western Blot analysis FGF2 Monoclonal Antibody (Product # 500-M38C-500UG) can be used at a concentration of 0.25-0.50 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human FGF-Basic is 0.25-0.50 ng/lane, under non-reducing conditions.

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产品信息

500-M38C-500UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse

分类

Monoclonal

类型

Antibody

抗原

E.coli derived, 17.2 kDa Recombinant Human FGF-basic (154 a.a.)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

纯化类型

Protein G

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2942198

产品详细信息

AA Sequence of recombinant protein: AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Preparation: Produced in BALB/c x ICR F1 mice using highly pure recombinant human FGF-basic. Anti-Human FGF-basic-specific antibody was purified from cell cµlture by Protein G affinity chromatography.

Sandwich ELISA: In a sandwich ELISA (assuming 100 µL/well), a concentration of 1.0-2.0 µg/mL of this antibody will detect at least 125pg/mL of Recombinant Human FGF-Basic when used with PeproTech's Biotinylated Antigen Affinity Purified Anti-Human FGF-Basic (500-P18BT) as the detection antibody at a concentration of approximately 0.25-0.50 µg/mL.

Western Blot: To detect Human FGF-Basic by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human FGF-Basic is 0.25-0.50 ng/lane, under non-reducing conditions.

靶标信息

FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Basic fibroblast growth factor; basic fibroblast growth factor bFGF; bFGF; FGF; fgf basic; FGF-2; FGF-b; Fibroblast growth factor; Fibroblast growth factor 2; fibroblast growth factor 2 (basic); H-FGF-b-147; H-FGF-b-154; HBGF-2; Heparin-binding growth factor 2; M-FGF-b; prostatropin

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基因别名: BFGF; FGF-2; FGF2; FGFB; HBGF-2

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UniProt ID: (Human) P09038

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Entrez Gene ID: (Human) 2247

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