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Invitrogen
FGF2 Monoclonal Antibody (2H5G2C11)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 8
FGF2 Antibody (MA5-15276) in ICC/IF
Immunofluorescence analysis of FGF2 was performed using 80% confluent log phase BJ and T-47D, control and PTI treated, cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FGF2 Monoclonal Antibody (2H5G2C11) (Product # MA5-15276) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Ale... View More
产品信息
MA5-15276
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
2H5G2C11
抗原
Purified recombinant fragment of FGF2 expressed in E. Coli.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
ascites
内含物
0.03% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_10983372
产品详细信息
MA5-15276 targets FGF2 in indirect ELISA, IHC and WB applications and shows reactivity with Human samples.
The MA5-15276 immunogen is purified recombinant fragment of FGF2 expressed in E. Coli.
靶标信息
FGF2 (FGFb, fibroblast growth factor basic) belongs to the fibroblast growth factor (FGF) family, and interacts with high-affinity transmembrane receptors to influence cell proliferation and tissue neovascularization. FGF2 exists as five isoforms with distinct intracellular localizations and functions. The 18 kDa isoform is predominantly cytosolic and acts through cell surface receptors, whereas the 22, 22.5, 24 and 34 kDa isoforms are nuclear and may signal independent of transmembrane receptor pathways. In humans, the gene is located on the q arm of chromosome 4. FGF2 has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for FGF2 contains multiple polyadenylation sites, and is alternatively translated from non-AUG and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. Diseases associated with FGF2 dysfunction include Kaposi Sarcoma and corneal neovascularization.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Basic fibroblast growth factor; basic fibroblast growth factor bFGF; bFGF; FGF; fgf basic; FGF-2; FGF-b; Fibroblast growth factor; Fibroblast growth factor 2; fibroblast growth factor 2 (basic); H-FGF-b-147; H-FGF-b-154; HBGF-2; Heparin-binding growth factor 2; M-FGF-b; prostatropin
基因别名: BFGF; FGF-2; FGF2; FGFB; HBGF-2
UniProt ID: (Human) P09038
Entrez Gene ID: (Human) 2247
protein tyrosine kinase activity
Ras guanyl-nucleotide exchange factor activity
fibroblast growth factor receptor binding
cytokine activity
protein binding
growth factor activity
heparin binding
1-phosphatidylinositol-3-kinase activity
ligand-dependent nuclear receptor transcription coactivator activity
chemoattractant activity
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
growth factor
intercellular signal molecule
MAPK cascade
activation of MAPK activity
branching involved in ureteric bud morphogenesis
positive regulation of endothelial cell proliferation
cell migration involved in sprouting angiogenesis
phosphatidylinositol biosynthetic process
chemotaxis
signal transduction
Ras protein signal transduction
nervous system development
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
organ morphogenesis
negative regulation of fibroblast migration
positive regulation of phospholipase C activity
regulation of phosphatidylinositol 3-kinase signaling
growth factor dependent regulation of skeletal muscle satellite cell proliferation
peptidyl-tyrosine phosphorylation
extracellular matrix organization
hyaluronan catabolic process
inositol phosphate biosynthetic process
somatic stem cell population maintenance
phosphatidylinositol-3-phosphate biosynthetic process
wound healing
positive regulation of cell fate specification
positive regulation of MAP kinase activity
positive regulation of blood vessel endothelial cell migration
negative regulation of blood vessel endothelial cell migration
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
regulation of angiogenesis
positive regulation of angiogenesis
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
embryonic morphogenesis
positive chemotaxis
release of sequestered calcium ion into cytosol
positive regulation of cell division
positive regulation of cardiac muscle cell proliferation
negative regulation of cell death
chondroblast differentiation
negative regulation of wound healing
positive regulation of ERK1 and ERK2 cascade
positive regulation of sprouting angiogenesis
regulation of endothelial cell chemotaxis to fibroblast growth factor
positive regulation of endothelial cell chemotaxis to fibroblast growth factor
