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Invitrogen
FIS1 Polyclonal Antibody
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
图: 1 / 11
FIS1 Antibody (PA5-53605) in ICC/IF
Immunofluorescence analysis of FIS1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FIS1 Polyclonal Antibody (Product # PA5-53605) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2... View More
产品信息
PA5-53605
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
偶联物
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 40% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2641645
产品详细信息
Immunogen sequence: MEAVLNELVS VEDLLKFEKK FQSEKAAGSV SKSTQFEYAW CLVRSKYNDD IRKGIVLLEE LLPKGSKEEQ RDYVFYLAVG NYRLKEYEKA LKYVRGLLQT EPQNNQAKEL ERLIDKAMKK D
Highest antigen sequence identity to the following orthologs: Mouse - 95%, Rat - 96%.
靶标信息
Fis1, a 17KDa integral membrane protein, is a novel member of mitochondrial fission machinery. It consists of a central leucine-zipper domain, a coiled-coil region, a tetratricopeptide repeat domain and a C-terminal transmembrane domain. It is a human orthologue of Fis1p (S.cerevisiae) and is localized in the outer mitochondrial membrane. Fis1 acts as a pro-apoptotic factor and plays a vital role in the regulation of mitochondrial fission. It interacts with DLP1 and Mdv1p and facilitates Dnm1p recruitment to the functional fission complex, thereby inducing apoptosis. Role of Fis1 has also been implicated in growth and division of peroxisomes. Loss of Fis1 inhibits apoptosis and results in elongation of mammalian mitochondria.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: FIS1 homolog; fis1 {ECO:0000250|UniProtKB:Q9Y3D6}; fission 1 (mitochondrial outer membrane) homolog; H_NH0132A01.6; hFis1; Mitochondrial fission 1 protein; mitochondrial fission molecule; rFis1; tetratricopeptide repeat domain 11; Tetratricopeptide repeat protein 11; TPR repeat protein 11
基因别名: 2010003O14Rik; CGI-135; FIS1; TTC11
UniProt ID: (Human) Q9Y3D6, (Rat) P84817, (Mouse) Q9CQ92
Entrez Gene ID: (Human) 51024, (Rat) 288584, (Mouse) 66437
mitochondrial fission
mitophagy
release of cytochrome c from mitochondria
protein targeting to mitochondrion
positive regulation of cytosolic calcium ion concentration
mitochondrial fusion
regulation of mitochondrion organization
peroxisome fission
negative regulation of endoplasmic reticulum calcium ion concentration
calcium-mediated signaling using intracellular calcium source
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
mitochondrial fragmentation involved in apoptotic process
protein homooligomerization
positive regulation of mitochondrial calcium ion concentration
mitochondrion morphogenesis
positive regulation of mitochondrial fission
positive regulation of protein targeting to membrane
positive regulation of intrinsic apoptotic signaling pathway
apoptotic process
