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产品信息
31687
应用
建议稀释比
已发表文章
产品规格
种属反应
Chemical
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
1F8-1E4
偶联物
激发/发射光谱
552/578 nm
查看光谱
形式
Lyophilized
浓度
1.7 mg/mL
纯化类型
purified
保存液
PBS, pH 7.6, with 15mg/mL BSA
内含物
0.05% sodium azide
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_908075
产品详细信息
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Fluorophore: Tetramethyl rodamine 5 (and 6)-isothiocyanate (TRITC)
Amax = 550 nm; Emax = 570 nm
Fluorophore/Protein Absorbance Ratio: A550/A280 = 0.44
Product #31687 is recommended for use in ELISA, immunocytochemical, immunofluorescent, immunohistochemical, immunoprecipitation and Western blot applications.
Restoration and Storage: Store product at 4°C until opened. Restore with 0.35 mL distilled water. Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. Product may be stored for up to 6 weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of 50% glycerol and store at -20°C; or as an alternative, aliquot without glycerol and store at -80º or below, avoiding repeated freezing and thawing.
Country of Origin: USA
靶标信息
FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Fluorescein
