Invitrogen

FLT3 Polyclonal Antibody

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3 篇参考文献

查看其他38个FLT3 (CD135)抗体

抗体检测数据 (2)
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FLT3 Antibody in Immunohistochemistry (IHC)

FLT3 Antibody in Immunohistochemistry (IHC)

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FLT3 Antibody (PA5-34448) in IHC

Immunofluorescence of FLT3 in human kidney tissue with FLT3 Polyclonal Antibody (Product # PA5-34448) at 20 µg/mL.

FLT3 Antibody in Immunohistochemistry (IHC)

FLT3 Antibody in Western Blot (WB)

FLT3 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP)

FLT3 Antibody in Western Blot (WB)

Product Image Gallery

FLT3 Antibody in Immunohistochemistry (IHC) — Immunofluorescence of FLT3 in human kidney tissue with FLT3 Polyclonal Antibody (Product # PA5-34448) at 20 µg/mL.

FLT3 Antibody in Western Blot (WB) — Western Blot analysis of FLT3 in 3T3 cell lysate with FLT3 Polyclonal Antibody (Product # PA5-34448) at (A) 1 and (B)2 µg/mL.

FLT3 Antibody in Western Blot, Immunocytochemistry, Immunoprecipitation (WB, ICC/IF, IP) — Figure 3 HFD activates FLT3/JAK3/STAT3 signaling on c-KIT + BM cells after 4 weeks. ( A ) Western blot showing increased phosphorylation of JAK3 (Y980/981) and STAT3 (Y705) among the c-KIT + MLL-AF9 knock in cells in BM, after 4 weeks of HFD. Data show mean +- SD; n = 3 mice per diet group; *** P < 0.001, two-tailed unpaired Student''s t-test; ns, non-significant. Grouping of blots cropped from different gels, see Supplementary Fig. S6 for full-length blots. Gel images were processed and analyzed for quantification (Fiji, NIH). ( B ) Western blot showing increased phosphorylation of FLT3 among the c-KIT + MLL-AF9 knock in cells in BM, after 4-weeks of HFD. After immunoprecipitation of pan tyrosine phosphorylated proteins with an anti-phosphotyrosine antibody (IP: pan Y), FLT3 was analyzed by a western blot (WB: FLT3). Increased phosphorylation of FLT3 following HFD was confirmed by immunoprecipitation with an anti-FLT3 antibody (IP: FLT3) followed by a western blot to detect pan phosphorylation of FLT3 (WB: pan Y). Data show mean +- SD; n = 3 mice per diet group; *** P < 0.001, two-tailed unpaired Student''s t-test. Grouping of blots cropped from different gels, see Supplementary Fig. S7 for full-length blots. Gel images were processed and analyzed for quantification (Fiji, NIH). ( C ) Flow cytometry on MLL-AF9 knock in BM cells showing median fluorescence intensity (MFI) for expression of FLT3 on Lin - SCA1 + c-KIT + (LSK) or Lin - c-KIT + (LK) cells. Data were analyzed usin

FLT3 Antibody in Western Blot (WB) — Figure 4 Quizartinib blocks the HFD-accelerated development of AML. ( A ) Experimental workflow describing the procedure. Image performed with the GIMP software (version 2.10.18, GIMP, https://www.gimp.org/news/2020/02/24/gimp-2-10-18-released/ ). ( B ) Western blot showing that a treatment with Quizartinib antagonizes the increased phosphorylation of FLT3 (pan tyrosine; panY) as well as JAK3 (Y980/981) and STAT3 (Y705) observed among the c-KIT + MLL-AF9 knock in BM cells after 4-weeks of HFD. Data show mean +- SD; n = 3 mice per diet group. Grouping of blots cropped from different gels, see Supplementary Fig. S8 for full-length blots. Gel images were processed and analyzed for quantification (Fiji, NIH). ( C ) Survival curves showing that mice fed a HFD and treated with Quizartinib survived longer than CD-fed mice, n = 7 mice per group; P value measured by Mantel-Haenszel test.

产品信息

PA5-34448

应用

建议稀释比

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免疫印迹 (WB)

1-2 µg/mL

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免疫组化 (IHC)

20 µg/mL

-

免疫细胞化学 (ICC/IF)

-

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免疫沉淀 (IP)

-

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产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

An 18 amino acid synthetic peptide near the center of human FLT3

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2551800

产品详细信息

A suggested positive control is NIH-3T3 cell lysate.

PA5-34448 can be used with blocking peptide PEP-1490.

靶标信息

FLT3 is a tyrosine protein kinase that is a member of the growth factor receptor tyrosine kinase family. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

High-fat diet intensifies MLL-AF9-induced acute myeloid leukemia through activation of the FLT3 signaling in mouse primitive hematopoietic cells.

Scientific reports

Hermetet F,Mshaik R,Simonet J,Callier P,Delva L,Quéré R

PA5-34448 was used in Western Blot, Immunocytochemistry to discover that consumption of a high-fat diet (HFD) accelerates the risk of developing acute myeloid leukemia (AML).

Wed Sep 30 00:00:00 EDT 2020

High-fat diet intensifies MLL-AF9-induced acute myeloid leukemia through activation of the FLT3 signaling in mouse primitive hematopoietic cells.

Scientific reports

Hermetet F,Mshaik R,Simonet J,Callier P,Delva L,Quéré R

PA5-34448 was used in Western Blot, Immunocytochemistry to discover that consumption of a high-fat diet (HFD) accelerates the risk of developing acute myeloid leukemia (AML).

Wed Sep 30 00:00:00 EDT 2020

High-fat diet intensifies MLL-AF9-induced acute myeloid leukemia through activation of the FLT3 signaling in mouse primitive hematopoietic cells.

Scientific reports

Hermetet F,Mshaik R,Simonet J,Callier P,Delva L,Quéré R

PA5-34448 was used in Western Blot, Immunocytochemistry to discover that consumption of a high-fat diet (HFD) accelerates the risk of developing acute myeloid leukemia (AML).

Wed Sep 30 00:00:00 EDT 2020

High-fat diet intensifies MLL-AF9-induced acute myeloid leukemia through activation of the FLT3 signaling in mouse primitive hematopoietic cells.

Scientific reports

Hermetet F,Mshaik R,Simonet J,Callier P,Delva L,Quéré R

PA5-34448 was used in Western Blot, Immunocytochemistry to discover that consumption of a high-fat diet (HFD) accelerates the risk of developing acute myeloid leukemia (AML).

Wed Sep 30 00:00:00 EDT 2020

High-fat diet intensifies MLL-AF9-induced acute myeloid leukemia through activation of the FLT3 signaling in mouse primitive hematopoietic cells.

Scientific reports

Hermetet F,Mshaik R,Simonet J,Callier P,Delva L,Quéré R

PA5-34448 was used in Western Blot, Immunocytochemistry to discover that consumption of a high-fat diet (HFD) accelerates the risk of developing acute myeloid leukemia (AML).

Wed Sep 30 00:00:00 EDT 2020

生物信息学

蛋白别名: CD135; CD135 antigen; Fetal liver kinase 2; Fetal liver kinase-2; FL cytokine receptor; FLK-2; FLT-3; Fms-like tyrosine kinase 3; fms-related tyrosine kinase 3; growth factor receptor tyrosine kinase type III; OTTHUMP00000214829; Receptor-type tyrosine-protein kinase FLT3; RP11-153M24.3; Stem cell tyrosine kinase 1; STK-1; tyrosine-protein kinase FLT3; Tyrosine-protein kinase receptor flk-2

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基因别名: B230315G04; CD135; FLK-2; FLK2; Flt-3; FLT3; Ly72; STK1; wmfl

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UniProt ID: (Human) P36888, (Mouse) Q00342

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Entrez Gene ID: (Human) 2322, (Mouse) 14255

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transmembrane receptor protein tyrosine kinase activity cytokine receptor activity vascular endothelial growth factor-activated receptor activity protein binding ATP binding protein complex binding glucocorticoid receptor binding protein homodimerization activity nucleotide binding protein kinase activity protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups ubiquitin protein ligase binding phosphatidylinositol 3-kinase binding transmembrane signal receptor

参与通路

leukocyte homeostasis myeloid progenitor cell differentiation pro-B cell differentiation transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of cell proliferation response to organonitrogen compound positive regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation cytokine-mediated signaling pathway hemopoiesis B cell differentiation organ regeneration common myeloid progenitor cell proliferation vascular endothelial growth factor signaling pathway positive regulation of tyrosine phosphorylation of STAT protein regulation of apoptotic process positive regulation of MAP kinase activity positive regulation of MAPK cascade positive regulation of phosphatidylinositol 3-kinase activity lymphocyte proliferation protein autophosphorylation cellular response to cytokine stimulus cellular response to glucocorticoid stimulus dendritic cell differentiation positive regulation of protein phosphorylation lymphoid progenitor cell differentiation pro-T cell differentiation protein phosphorylation negative regulation of cell proliferation phosphorylation lymphocyte differentiation cell differentiation negative regulation of B cell differentiation