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Immunohistochemistry analysis of FNDC3A in paraffin-embedded zebrafish tissue. Samples were heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4, 20 minutes) and blocked in 5% BSA (30 min, room temperature), incubated with FNDC3A polyclonal antibody (Product # PA5-109309) at a dilution of 1:200 (30 min, room temperature), and followed by HRP conjugate, DAB and hematoxylin (mounted with DPX).
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