Invitrogen

FTO Polyclonal Antibody

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FTO Antibody (PA5-120656) in WB

Western blot analysis of FTO in extracts of various cell lines. Samples were incubated with FTO Polyclonal antibody (Product # PA5-120656) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

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产品信息

PA5-120656

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of zebrafish FTO (XP_009301626.1).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2914228

产品详细信息

Positive test controls include: Mouse brain, Rat brain.

Immunogen sequence: MKPRQRKQYF RNMKRSDDSE REKRRKRRRL LQELGEQRIP YLSPTDPGFQ DLWDSSYAGL ALRQSGTLPE GLHEKVQSAL LTLQRHGCLL RDLVRVRDRD VFTAVSRALV GQPGYTYRYL DTRLFTIPWH CEGEEGQKDE KGKPCCDSDL RDACKALWEL NQFFCSDVKQ QTNARGVKRT RSDTENSEDA PGEGMCEEES VKDRLVEEKT IEEEEEDSGQ GCSHSSPPSS TPPAAQASLV QFNVTLINYM NPAAMTQLKE EPYYGMGKMA VGWHHDENLV PLSPVAVYSY SCPAEPKNEG VTEKDGEGKS KEKEREAKGE GTSTEEVKKE GEASVKEDVE KEKTCWRVG

靶标信息

FTO is a novel protein and a member of the non-heme dioxygenase (Fe(II)-and 2-oxoglutarate-dependent dioxygenases) superfamily. Amino acid conservation patterns support this hypothesis and indicates that both 2-oxoglutarate and iron should be important for FTO function. Though not much is yet known about FTO, it is thought that the protein may somehow modify the activity of genes involved in metabolism and fat storage, which in turn may influence a person's risk of obesity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: AlkB homolog 9; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein; fatso; m6A(m)-demethylase FTO; MGC5149; mRNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; mRNA N(6)-methyladenosine demethylase FTO; Protein fatso; protein fto; tRNA N1-methyl adenine demethylase FTO; U6 small nuclear RNA (2'-O-methyladenosine-N(6)-)-demethylase FTO; U6 small nuclear RNA N(6)-methyladenosine-demethylase FTO

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基因别名: ALKBH9; AW743446; BMIQ14; FTO; GDFD; KIAA1752; mKIAA1752; RGD1305121

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UniProt ID: (Human) Q9C0B1, (Rat) Q2A121, (Mouse) Q8BGW1

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Entrez Gene ID: (Human) 79068, (Rat) 291905, (Mouse) 26383

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